Mouse Restraint Device October 8, 2009 Eamon Bernardoni, Vanessa Grosskopf, Jim Mott, Samantha Paulsen, Brooke Sampone Problem Statement: The Small Animals Imaging Lab located in the Wisconsin Institutes for Medical Research is in need of a solid, adjustable device that will restrain mice during Positron Emission Tomography and Computerized Tomography (PET/CT) scans. These scans are used to pinpoint cancerous growths within the animal prior to treatment. The device must restrain the animal’s extremities to prevent movement and must include a quantitative analysis device to replicate the animal's exact anatomical position. The device should improve efficiency for animal restraint compared to the current cardboard restraint device. Client Requirements: • Adjustable device to fit mice and accommodate 20-50 grams • Device should be 5”- 6” in length to fit a mouse • Device should not interfere with nose cone • Device should include measurement device to replicate exact position of mouse • Material should not interfere with imaging from PET/CT machine and should not include cloth • Method of restraining animal should take no longer than 10 minutes • Device should prevent the animal from leaving the bed in the event that it wakes up • Must attach to carbon fiber bed Design Restraints: 1. Physical and Operational Requirements a. Performance requirements: The device should accommodate mice ranging in size from 5”-6” in length and 20-50 grams. The mouse should be restrained in less than 10 minutes. 3-10 mice will be scanned several times over a period of roughly two weeks. 100 animals per year are scanned by the PET/CT machines. The device should be securely attached to the bed. b. Safety: The device should conform to RARC and lab protocol. No animals should be harmed by the device. Absorbent material should not be used as to prevent retention of radioactive substances. c. Accuracy and Reliability: The device should allow for the exact alignment of the anatomical position of each mouse over the duration of the study within 1 mm of the original position of the animal. Lasers are used to help align the position of the animal once it is attached to the bed. d. Life in Service: The device will be used for approximately 400 hours each year. e. Shelf life: If sliders are incorporated in the design, lubrication of the device may be necessary. Depending on the type of attachment, attachment material may need to be replaced once worn. f. Operating Environment: The device will be used in the research laboratory. There may be corrosion of materials due to lubrication of the device and radioactive liquids.g. Ergonomics: The device should be attached with minimal effort in a matter of 5-10 minutes. The size settings should not cause eye strain. Animal position should be easy to replicate. h. Size: The device should be no more the 0.5” thick or 3” wide. The length should not exceed 12”. i. Weight: The device weight should be less one pound. j. Materials: The materials used in the device should not interfere with the imaging procedures. Cloth should not be used. Carbon fiber would be the preferred material. k. Aesthetics, Appearance, and Finish: The device should be neutral in color, smooth, and have no sharp edges. 2. Product Characteristics a. Quantity: The client requires one device. b. Target Product Cost: $100 3. Miscellaneous a. Standards and Specifications: The device must comply with RARC and lab protocol for animal safety. b. Customer: The device will be used in the Small Animal Imaging Lab at UW-Madison for PET/CT scans. c. Patient (animal)-related concerns: The device must be wiped down between animals. d. Competition: Due to the fact that the device is custom to this specific research lab, there is no foreseen
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