Biological Imaging Chamber Andrew Dias, Justin Lundell, Val Maharaj, Jeremy Schaefer, Michael Socie Client: Lance Rodenkirch Advisor: Bill Murphy Last Updated: 9/14/07 Function Construct a live cell imaging chamber to be used for laser-based confocal and multiphoton imaging. Device needs to control temperature and gas-environment inside as well as enable the use of perfusion. Client Requirements - Maintain environment with 95% air, 5% CO2 - Device must allow control of air temperature - Allow perfusion to sample - Mechanism for control of X, Y, theta of cell culture Design Requirements 1. Physical and Operational Characteristics a. Performance Requirements: Imaging chamber should allow live cell imaging to occur in a controlled environment. Gas make-up, gas temperature, and perfusion need to be controlled. b. Safety: Chamber must not damage microscope or surrounding equipment in the client’s lab. Use of pressurized gas tanks including CO2 needs to be done in a safe manner. c. Accuracy & Reliability: pH level must be maintained between 6-8 in culture media. CO2 level must remain close enough to 5% to maintain cell life. Testing will show what level is too high. d. Life in Service: e. Shelf Life: Chamber itself will not degrade with time. Shelf life of whole system will depend on size of input gas tank and flow rates which will be determined by testing. f. Operating Environment: Chamber will be used with an Inverted Nikon TE2000 U microscope in W.M. Keck Laboratory on the UW campus. g. Ergonomics: Chamber must allow for easy-access to put in and remove samples. h. Size: Must fit on moveable XY stage in between lens and base of microscope. 30 x 27.6 x 3 cm. i. Weight: Must not damage microscope stage. j. Materials: No plastic in microscope image field. k. Aesthetics, Appearance, & Finish: Chamber must be easy to sterilize. Good organization of peripheral tubes, etc.2. Production Characteristics a. Quantity:1 b. Target Production Cost: $500 3. Miscellaneous a. Standards & Specifications: N.A. b. Customer: N.A. c. Patient-related Concerns: N.A. d. Competition: Incubator 2000; 20/20 Technology, Inc. Incubator 2000 is a miniature imaging chamber with control for temperature, humidity, and atmosphere. PRICE Focht Chamber System 3: Bioptechs, Inc. FCS3 is a live cell imaging chamber with control for gas flow speed, temperature, and gas make-up as and is perfusion compatible. The FCS3 system starts at
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