BBMMEE 220000//330000 ((BBiioommeeddiiccaall EEnnggiinneeeerriinngg DDeessiiggnn)) FFaallll 22000088 PPrroojjeecctt ##2266:: DDeevveellooppmmeenntt ooff aa DDeevviiccee ffoorr NNeeuurroocchheemmiiccaall SSaammppllee CCoolllleeccttiioonn ffrroomm FFrreeeellyy MMoovviinngg MMoonnkkeeyyss FFIINNAALL RREEPPOORRTT DDeecceemmbbeerr 1122,, 22000088 TTEEAAMM MMEEMMBBEERRSS:: PPaauull SScchhiillddggeenn ((TTeeaamm LLeeaaddeerr)) AAnnggwweeii LLaaww ((CCoommmmuunniiccaattoorr)) LLaauurreenn EEiicchhaakkeerr ((BBSSAACC)) CCoollee DDrriiffkkaa ((BBWWIIGG)) CCLLIIEENNTT:: DDrr.. EEii TTeerraassaawwaa DDeeppaarrttmmeenntt ooff PPeeddiiaattrriiccss,, UUWW--MMaaddiissoonn AADDVVIISSOORR:: PPrrooffeessssoorr JJoohhnn WWeebbsstteerr DDeeppaarrttmmeenntt ooff BBiioommeeddiiccaall EEnnggiinneeeerriinngg,, UUWW--MMaaddiissoonnBBMMEE 220000//330000 ((BBIIOOMMEEDDIICCAALL EENNGGIINNEEEERRIINNGG DDEESSIIGGNN)) MMIIDD--SSEEMMEESSTTEERR RREEPPOORRTT AADDVVIISSOORR:: PPRROOFFEESSSSOORR JJOOHHNN WWEEBBSSTTEERR PPRROOJJEECCTT ##2266 ((NNEEUURROOCCHHEEMMIICCAALL__SSAAMMPPLLIINNGG)) Page 2 of 21 LLAASSTT UUPPDDAATTEEDD:: October 22, 2008 TTaabbllee ooff CCoonntteennttss Abstract 4 Problem Motivation 4 Background Rhesus Macaque 4 Client’s Research 5 Microdialysis & Experimental Procedure 5 Client’s Requirements & Design Constraints 6 Last Semester’s Design 6 Design Alternatives for Cylindrical Top Aluminum 7 Porous Aluminum 8 Plastic 8 Design Matrix for Cylindrical Top 9 Design Alternatives for Strap Configuration Lock & Key 10 Zip Tie 11 Belt 11 Design Matrix for Strap Configuration 11 Proposed Solution 12 Final Design 12 Weights 13 Testing 13 First Live Testing 13 Second Live Testing 14 Testing Conclusions 15 Future Work 15BBMMEE 220000//330000 ((BBIIOOMMEEDDIICCAALL EENNGGIINNEEEERRIINNGG DDEESSIIGGNN)) MMIIDD--SSEEMMEESSTTEERR RREEPPOORRTT AADDVVIISSOORR:: PPRROOFFEESSSSOORR JJOOHHNN WWEEBBSSTTEERR PPRROOJJEECCTT ##2266 ((NNEEUURROOCCHHEEMMIICCAALL__SSAAMMPPLLIINNGG)) Page 3 of 21 LLAASSTT UUPPDDAATTEEDD:: October 22, 2008 Ethical Considerations 16 References 17 Appendix 1: Device Assembly Protocol 18 Appendix 2: Product Design Specifications 19 Appendix 3: Project Expenditure 21BBMMEE 220000//330000 ((BBIIOOMMEEDDIICCAALL EENNGGIINNEEEERRIINNGG DDEESSIIGGNN)) MMIIDD--SSEEMMEESSTTEERR RREEPPOORRTT AADDVVIISSOORR:: PPRROOFFEESSSSOORR JJOOHHNN WWEEBBSSTTEERR PPRROOJJEECCTT ##2266 ((NNEEUURROOCCHHEEMMIICCAALL__SSAAMMPPLLIINNGG)) Page 4 of 21 LLAASSTT UUPPDDAATTEEDD:: October 22, 2008 AAbbssttrraacctt The goal of this design project is to develop and construct a device to provide protection for the microdrive unit, an apparatus that is used in microdialysis experiments conducted on nonhuman primates. This would allow the experiments to be conducted without restraining the monkeys in a chair, providing them with a more comfortable and realistic experimental setting. Currently, there are no existing devices in the market specifically catered for this purpose. Building on last semester’s prototype, the current design consists of an aluminum casing with a form fitted silicone rubber interior to surround the microdrive unit. In accordance with the client’s requirements, the team considered alternatives to make the device more lightweight and secure, and decided to pursue a porous aluminum cylindrical top as well as a zip tie strap configuration to accomplish this aim. The team implemented these improvements and then carried out preliminary testing on live monkeys. Results of the testing importune the need for further modifications to be made, particularly to reduce the weight of the device. PPrroobblleemm MMoottiivvaattiioonn Microdialysis allows for real-time in vivo measurements of various substances in the body2. In particular, direct measurements of neurochemical substances in the brain from free-moving nonhuman primates is significantly important for understanding complex brain function and developing treatment strategies for brain disorders in humans. A modified microdialysis method has been developed for application to Rhesus macaques (Macaca mulatta). However, it requires restraining the monkeys in chairs for up to 12 h while samples are collected. Although this is considered a norm and the monkeys have been appropriately conditioned, the situation is less than ideal since the monkeys are subjected to a certain amount of discomfort due to the restricted movement and long period of chairing. The data collected might also not be representative of a monkey in its natural environment, since it is confined to a chair. One way to address this problem is to allow the monkeys to be free from chairing while the experiments are being conducted. Besides alleviating the discomfort experienced by the monkeys, it would also better simulate the monkey’s natural environment since it would be free to move around, albeit within the confines of a cage. Additionally, this method would allow the experiments to be conducted without the 12 h limit imposed by chairing. More time would be available for sample collection and safety inspections to be conducted, allowing the experiments to be carried out more smoothly and safely. However, allowing the monkeys to move freely introduces the risk of them tampering with the experimental apparatus (called a microdrive unit), thus compromising the experimental procedure. Hence, a protective device must be constructed to protect the microdrive unit during the experiment. This device must be compatible with the monkeys such that it will not incur greater discomfort than is necessary or encumber the experimental process. There are currently no products in the market that specifically address this issue, so a suitable device must be designed and constructed. BBaacckkggrroouunndd RRHHEESSUUSS MMAACCAAQQUUEE The Rhesus macaque, also known as the Rhesus monkey, is one of the best known species of Old World monkeys. Rhesus macaques have an extensive geographic distribution and are found ubiquitously throughout mainland Asia, ranging from Afghanistan to India and Thailand to southern China. They range in color from brown to gray and have little fur, if any, on their reddish-pink faces5. On
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