Mouse Positioning Device for Longitudinal Cancer ResearchJustin Jeffery, Lab ManagerJamey Weichert, PhDSmall Animal Imaging Lab, UW Carbone Cancer CenterWalter Block, PhDWalter Block, PhDEamon BernardoniVanessa GrosskopfJim MottSamantha PaulsenBrooke SamponeCancer Treatment: Lab Productivity• Longitudinal cancer treatment development– Animal models– Diagnostic imaging methods– Limitations in productivity• Improved productivity requirements–Repeatable imaging methods –Repeatable imaging methods – Current methods• Improved productivity options• Mouse positioning device selection– Testing– Potential problems– Future workEvaluating New Cancer Treatments• Provide state-of-the-art, noninvasive imaging support• Small Animal Imaging Lab provides images for major producers of microCT, microPET, and hybrid scanners.• Use animal models to perfect imaging techniqueshttp://www.df.unipi.it/~fiig/research_reconstruction.htmhttp://www.cancer.wisc.edu/uwccc/services_smallanimal_equip.aspReasons for a New Device• Small Animal Imaging Lab• Restrain mice during scans• PET/CT modality• Precise repositioning •Required for serial scans•Required for serial scans• Registration processing is time consuming• Current method is labor intensivehttps://www.medical.siemens.com/Design Criteria• Restrains mouse in case anesthesia fails• Replicates position within 1 mm• Positioning of animal and device takes 5-10 min• Minimize interference with PET/CT imaging• Easy to clean, no permanent cloth• Acceptable for RARC approval• Attaches to existing carbon fiber bed • Multiple scans of 3-10 mice over multiple 2 week periods• $100 budgetCurrent Device in Small Animal Imaging LabDevices Used in Other LabsCrump Institute for Molecular Imaginghttp://www.iop.org/EJ/article/Numirahttp://www.numirabio.com/site/services/imaging-chamberm2mhttp://www.m2mimaging.com/products/http://www.iop.org/EJ/article/Sliding Velcro Slot Design• Dimensions: 5 1/2” × 3 1/2” × 1/8”• Material: Acrylonitrile butadiene styrene (ABS)• Inexpensive• Has low attenuation• Two 1 1/2” slits in each corner cut 1/4” apart• Restraints: 4 double-sided Velcro strips•Ruler•Ruler• Device attaches to bed with pegs on bottom of boardLego Board Design• Dimensions: 5 1/2” x 3 1/2” x 1/8”• Material: LEGO - ABS [1]• 144 1/5” diameter raised cylinders – Aligned in 16 rows and 9 columns– Letter and number coordinate system• Device attaches to the bed with pegs on bottom of board•Two LEGO pegs attached with a band placed over limbs•Two LEGO pegs attached with a band placed over limbs• Pegs also attached around body• Record coordinates of pegs to replicate positionPeg Board Design• Dimensions: 5 1/2” × 3 1/2” × 1/8”• Material: ABS• 459 1/16” holes drilled 1/8” apart, 1/4” border – Aligned in 27 rows and 17 columns – Letter and number coordinate system• Device attaches to bed with pegs on bottom of board•Flat board allows for tracing of body of mouse•Flat board allows for tracing of body of mouse• Pegs attached to bands to go over limbs of miceEvaluation of Design AlternativesCriteria Velcro SlotLego BoardPeg BoardAccuracy (35) 20 25 33Ease of Use/Speed of Attachment (20)12 18 16Animal Safety (15) 12 12 10Feasibility (15) 10 14 12Sterility (10) 8 9 10Cost (5) 3 5 5TOTAL (100) 65 83 86Obstacles to Overcome• Ergonomics• Acquiring peg materials• Fabrication of pegs• Durability of pegs• Scanner friendly adhesiveshttp://z.about.com/d/familycrafts/1/0/K/o/1/ct2-16_superglue.jpghttp://www.ellsworth.com/imagelibrary/jpegs/100/LargePics/4707GL.jpgHow will we test our device?• Repeated attachments and scans• Scanning device materialsWhere will we go from here?• Determine which formula of ABS to use [2]– 15% to 35% acrylonitrile– 5% to 30% butadiene–40% to 60% styrene–40% to 60% styrene• Determine what type of bands and pegs to use• Secure device attachments to bed at WIMRReferences[1] The LEGO Group. (2009). Product quality and safety. Retrieved from http://www.lego.com/eng/info/default.asp?page=safety[2] Lenau, Torben. (2003). Material ABS – acrylonitrile butadiene styrene. Retrieved from http://designinsite.dk/htmsider/m0007.htmAcknowledgements• Justin Jeffery• John Floberg• Jamey Weichert• Walter
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