BIOLOGY 263, FALL 2009 FINAL EXAMINATION Name Date: Laboratory Practical Stations. 1. a. 7. a. b. b. 2. a. 8. a. b. b. 3. a. 9. a. b. b. 4. a. 10. a. b. b. 5. a. 11. a. b. b. 6. a. 12. a. b. b. 1000x mag. 13. a. Domain? 14. a.&b. Use two correct technical terms to describe b. Shape term? this leaf.Definitions. Define each BIOLOGICAL term from THIS LABORATORY in an accurate, concise, and lucid manner. 1. Bacterial Colony: 2. (Paper) Chromatography: 3. Electrophoresis: 4. Gram Stain: Hypothesis Evaluation and Understanding Experimental Design. Hypothesis = Women who use makeup will have greater microbial species richness on their facial skin than women who do not wear makeup. Data = Mean number of species per cm2 skin in women who wear makeup = 5.3, Mean number of species per cm2 skin in women who do NOT wear makeup = 6.3, p value = 0.154 Circle one: Hypothesis Supported Hypothesis NOT Supported (Rejected) What is the treatment in this study? What is the negative control in this study? Data Analysis and Interpretation. Given the following data from a small, reproductively isolated, constant-sized population, a) calculate the allele frequencies for every time period. individuals Allele freq. gener-ations BB Bb bb fB fb 0 72 96 32 5 20 90 90 10 0 30 170 15 0 0 200 20 0 0 200 b) Create a properly labeled graph of the allele frequency of one of the alleles (NOT both) over generations.c) Is the population at Hardy Weinberg equilibrium at time 0? (show calcs.) d) Propose a 1 sentence plausible evolutionary explanation for what you observed above. 4. Create a phylogenetic tree based on the followings set of data. Be certain to indicate derived characteristics (Synapomorphies) on the tree. (6%) head color Repro-duction Tail color Eye shape Nostril size Doofuses red frequent yellow round small Dorkuses red frequent yellow round small Goofuses blue rare yellow oval small Dweebuses blue rare yellow oval small Geekuses - OUTGROUP red rare green oval large 5. Study Design. Use pages in your bluebook or greenbook to design and describe a simple but rigorous experiment to test the hypothesis that penicillin does not inhibit the bacterium Escherichia coli. Briefly describe how you would design the study to test this hypothesis. (What would you do and what would you use? Assume you can manipulate this situation significantly.) What quantitative (numerical) data you would gather and
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