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MSU MMG 301 - Module 21

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Module 21If given a diagram of the individual components of viruses; including nucleic acid, capsomeres and capsids and envelope, assemble these into a complete virus; where does the envelope originate from Order the steps in the life cycle of a virus that causes lysis of its host cell Attachment, penetration of viral nucleic acid, synthesis of viral nucleic acid and protein, assembly and package of new viruses, cell lysis and release of new virionsCreate a graph showing liquid culture turbidity vs time for a lytic virus infection; also do this for virus count vs time What is a virus “titer” and how is it determined; how is a plaque formed on an agar plate A virus “titer” is the concentration of a virus. Each plaque originates from a single infected cell.Describe how are T4 viral components made precisely at the time they are neededT4 viral components made precisely at the time they are need by synthesis of different genes are timed so that the correct viral components are available to assemble the virus.Distinguish the lytic and lysogenic paths that an E. coli cell can take after it is infected with bacteriophage lambda Lytic- virus replicates and lyses the hostLysogenic- virus DNA genome incorporate into the host chromosome.Create a diagram that illustrates induction of a prophage going to cell lysis How do viruses efficiently use of their limited genome size How viruses efficiently use of their limited genome size by overlappingKnow the possible outcomes of infection by certain animal viruses and what “transformation” isThe possible outcomes of infection by certain animal viruses and what transformation is tumor cell division.What replication enzyme is a distinguishing feature of retroviruses; what are the three major gene regions in retroviruses; what are oncogenes The replication enzyme that is distinguishing feature of retroviruses reverse transcriptase. Three major gene regions:Gag- core and capsid structural proteinsPol- reverse transcriptase, integrase, proteaseEnv- envelope proteinsOrder the steps of the retrovirus life cycle, what type of genome, dsDNA intermediate, why are protease, reverse transcriptase, and fusion inhibitors helpful in treatment of HIV 1. Entry and uncoating of the retrovirus2. Reverse transcriptase activity 3. Viral DMA enters nucleus and integrates into the host genome4. Transcription by host RNA polymerase forms viral mRNA and genome copies5. Translation of mRNA forms viral proteins; new nucleocapsids assembled and releasedby buddingBe able to diagram the step of retroviral (and other enveloped viruses) release from host cell by

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