Module 16Summarize how advancements in sequencing have led to changes in microbiologyAllow genomes of individual microorganisms or entire microbial communities to be determined.Describe the process of determining a bacterial genome or set of genomes, transcriptome and proteomeSummarize the process of gene annotation1. Isolate nucleic acid from sample2. Sequencing by synthesis obtain small sequencing read3. Assemble small sequences into larger contigs4. Use PCR and side specific sequence to fill in any gaps5. AnalysisIndicate how genomics technologies can assist in understanding bacterial evolutionCompare the information determined by genomics, metagenomics, transcriptomics and proteomicsMetagenomics- examines the metabolic products of an organizationTranscriptomic- study of the whole transcriptome changes across a variety of biologic conditionsProteomics- looks at protein products of genesChoose which of the above approaches is most useful for a given question or experimentUse example data to make predictions about a given organisms lifestyle or
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