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MSU MMG 301 - Module 19

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Module 19Describe the difference between generalized and specialized transduction, including what DNA is transferred from donor to recipient Generalized transduction- random piece of DNA then accidently packaged into virus.Specialized transduction- DNA that is adjacent to the prophage can be transferred to a recipient cell.Explain the features of a conjugative plasmid and what a pilus is and what is its function The features of a conjugative plasmid is it transfers a copy of itself into a recipient cell. A pilus is a protein tube it attaches its self to the recipient cell and transfers its genes. List the important characteristics of a plasmid Important characteristics of a plasmid are double stranded circular DNA, few thousand BP, 1 copy/cell, replicate separate chromosomes.Diagram or explain the two mechanisms of F plasmid transfer in E. coli – either when a separate plasmid or integrated into a host chromosome F plasmid conjugation can involve 1) transfer of a copy of the plasmid DNA 2) integration into cell chromosomes followed by chromosome transferExplain what an Hfr strain is and how the F plasmid gets inserted into the chromosome The Hft strain is when the F plasmid can integrate into the genome. Homologous recombination results in insertion of the F plasmid into the chromosome. Describe a transposable element and the two types there are; briefly describe the two mechanisms of transposition Transposable elements are segments of DNA that can copy or move from one location within a cell’s genome to another location. Two mechanisms of transposition are conservative and replicativeExplain the main purpose of the CRISPR system; how does it protect a cell from viruses or plasmids; what is the role of the Cas proteins The main purpose of CRISPR system is to cut out individual segments of RNA that are incorrect and replace them with the correct ones. It protects a cell from viruses or plasmids is with Cas proteins. The Cas proteins target incoming DNA for

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