ADSC 2010 Lecture 29Outline of Last Lecture I. Estrus SynchronizationA. BenefitsB. DisadvantagesC. Methodsa. Cowsb. Ewesc. Sowsd. MaresII. Artificial Insemination A. Benefits Outline of Current Lecture I. Artificial InseminationA. Time for Breedinga. Cowb. Ewec. Sowd. MareII. Embryo TransferA. BenefitsB. LimitationsC. ProcessIII. Pregnancy DetectionA. CowB. EweC. SowD. MareCurrent Lecture: I. Artificial InseminationA. Time for Breedinga. Cow- Breed 12 hours after standing estrus- AM/PM RULE: Detect in am/breed in pm (vise versa)b. Ewe- Breed 12 hours after standing estrusc. Sow- If checked one time, breed when detected and 24 hours later- If checked twice, breed 12 hours after detection and 12 hours laterd. Mare- Breed every other day beginning the second day of estrusII. Embryo TransferA. Benefits- Increases production from superior females- Allows greater number of offspring from a particular matingB. Limitations- Cost- Management requirements- Requires AI and estrus synchronizationC. Process- Superovulate genetically superior female (DONOR); FSH and GnRH- AI to generally superior male- Collect embryos (FLUSHING: before attachment) and evaluate for viability- Store viable embryos- Synchronize the RECIPIENT female - Implant embryos into RECIP and allow RECIP to raise the offspring- Cows normally produce 7-10 calves over their lifetime- 150,000 ova in cow’s ovary can be brought out through superovulation III. Pregnancy DetectionA. Cow- Rectal palpation around 35-40 days gives >95% accuracy- Ultrasound diagnosis can be done earlier- Black circle: blastocyst formingB. Ewe- No simple, early detection methodC. Sow- Ultrasound diagnosis at 30-80 Days gives about 90-95% accuracyD. Mare- Rectal palpation at 30-45 Days- Ultrasound diagnosis can be done earlier- Immunological testing for PMSG from day
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