MMG301 1nd Edition Lecture 26 Outline of Last Lecture I. Biotechnology part 1Outline of Current Lecture II. Microbial productsIII. Strain optimizationIV. Microbial impacts on petroleumCurrent Lecture-microbial products-commodities:-food additives – amino acids, thickening agents, vitamins-solvents – butanol, ethanol-biofuels – ethanol, methane, hydrogen-processes:-sewage treatment-bioleaching – extraction of metals from ore-biocatalysis – production of chiral compounds-fine chemicals: -pharmaceuticals – antibiotics, antifungals-laboratory and diagnostic reagents – enzymes biochemicals, proteins-emerging technologies-DNA and protein array technology-biosensors-strain optimizationThese notes represent a detailed interpretation of the professor’s lecture. GradeBuddy is best used as a supplement to your own notes, not as a substitute.-genetic methods plasmid cloning of antibiotic production genes and/or-optimization of growth conditions to obtain high-yielding variants-regulatory mutants: select mutants that have lost normal feedback inhibition of biosynthetic pathway-example: lysine production by Brevibacterium flavum-microbial impacts on petroleum-undesired microbial degradation problems-in storage tanks, microbes need water, so organisms grow at oil-water interfaces; under anoxic conditions hydrogen sulfide (H2S) is produced, which is corrosive;-biofilms can plug rock pores and reduce fluid flow in petroleum recovery-beneficial actions of microbes -microbes can aid in petroleum recovery-production of gasses in oil formations to increase reservoir pressure-production of extracellular surfactants that decrease water surface tension in oil deposits-bioremediation of petroleum spills-many microorganisms metabolize various components of petroleum and oxidize them to CO2: some produce extracellular surfactants and emulsifying agents-remediation is promoted by 1. application of petrophilic microbial suspensions2. application of growth-promoting
View Full Document
Unlocking...