1. Fluorescence microscopy: A. Is used to detect Gram-stained bacteria. B. Produces high contrast 3-D like images. C. Is generally used for extremely thin cells such as Treponema pallidum. D. Can be used with certain unstained cells as well as with specially stained cells. E. Requires staining with electron-dense metal ions. 2. According to the following multiple alignment of partial rRNA sequences, isolates C & D are identical. Which of the remaining isolates is closest to the C & D pair? Isolate A AAUCUGGGUCUA Isolate B AAACCGGGUCUA Isolate C AAAUCGUGGCUA Isolate D AAAUCGUGGCUA Isolate E AAAUCGAGGCUA A. A B. B C. E D. A & B are equally distant to C & D E. B & E are equally distant to C & D3. The high-temperature limit to life is probably governed by: A. The rate of protein denaturation (precipitation). B. The decomposition (hydrolysis) of nucleotides such as ATP. C. The unraveling of double-stranded DNA. D. Disintegration of the cell walls. E. Thermal instability of the nuclear membrane. 4. The late 1800’s were a particularly exciting time in Microbiology with many important medical and ecological discoveries. Which of the following events did not occur in the 1880-1900 time period? A. Identification of the microbes associated with tuberculosis, diptheria, typhoid fever, gonorrhea, bubonic plague, and several other human diseases. B. Development of methods to grow pure cultures of bacteria on solid medium. C. Discovery of penicillin, the first known antibiotic, by Alexander Fleming. D. Identification of the central role of microbes in the nitrogen cycle. E. Discovery of “autotrophic” bacteria that use CO2 as their sole carbon
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