MMG301 1nd Edition Lecture 24Outline of Last Lecture I. Types of mutationsII. DNA repair of certain mutationsIII. Auxotroph mutantsIV. Gene transfer by transformation and transductionOutline of Current Lecture II. ConjugationIII. Gene mappingIV. Regulation of cell pathwaysV. Allosteric regulation and controlCurrent Lecture-conjugation: direct plasmid transfer or integrated plasmid-mediated chromosome transfer-in E. coli, the F (fertility) plasmid is involved. -cells having the F plasmid are designated F+-F plasmid encodes the pilus + other genes -F+ cells donate DNA through the sex pilus -the F plasmid can exist either of two states:-as a separate plasmid-incorporated into the cell genome-an F plasmid that is incorrectly excised so that it carries some chromosomal genes is termed an F’ (F-prime) plasmid-plasmid-mediated transfer of genes from one cell to another is also termed mobilization: genes adjacent to the integration site are moved from the donor cell to the recipient cell-a method called Interrupted Mating used Hfr genome transfer to map the relative location of genes on a genomeThese notes represent a detailed interpretation of the professor’s lecture. GradeBuddy is best used as a supplement to your own notes, not as a substitute.-merodiploid: two copies of a gene within a single cell-a second copy of a gene is carried on a transducing phage-a second copy of a gene is carried on a F plasmid -gene: segment of DNA that codes for a polypeptide, tRNA, or rRNA-operon: contiguous set of genes transcribed as a single mRNA under the control of a single regulatory region-regulon: spatially separated genes regulated by a common regulatory molecule-sigma factor: protein that recognizes specific DNA promoter site; aids binding of RNA polymerase; is released as transcription begins-repressor: dimeric protein binds to symetric inverted repeat sequences on
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