MMG301 1nd Edition Lecture 15Outline of Last Lecture I. Bacterial internal structuresOutline of Current Lecture II. Microbial nutritionIII. CultivationIV. Enrichment Current Lecture-70-80% of microbial cell mass is water-95% of dry cell weight is macroelements-C, H, O, N, S, P, K, Mg, Ca-carbon is need in the largest amount-nitrogen is the second largest-inorganic nutrients:-inorganic macronutrients: K, Mg, P, S, Ca – needed for many cell processes-inorganic micronutrients: Cr, Co, Cu, Mo, Zn, others-synthetic (defined) medium: component molecules are known-minimal medium: contains only those ingredients required for growth of a given organism-complex (undefined) medium: ingredients of undefined composition-selective medium inhibits the growth of certain microbes but allows others to grow-differential medium indicates whether certain chemical reactions occur-sterilization: the complete killing or removal of all viable organisms -culture media are sterilized primarily by autoclaving or filtrationThese notes represent a detailed interpretation of the professor’s lecture. GradeBuddy is best used as a supplement to your own notes, not as a substitute.-autoclaving: steam under high pressure, at 121°C (250 F)-heat-sensitive liquids: sterilized by filtration-reusable membrane filter apparatus-disposable pre-sterilized membrane filter unit-pure culture: population derived from a single
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