Bioreactor for Tissue Engineering Team Bioreactor Week 14 April 26 to May 2 2007 Team Members Karen Chen Team leader Richard Bamberg Communications Rachel Mosher BSAC Dustin Gardner BWIG Client Dr Susan Thibeault Dept of Otolaryngology UW Medical School Phone 608 263 6751 Email thibeault surgery wisc edu Advisor Brenda Ogle Assistant Professor Dept of Biomedical Engineering Phone 608 265 8267 Email ogle wisc edu Problem Statement The aim of this project is to redesign and reproduce a pre existing Bioreactor that is specific to the culturing of human vocal fold fibroblasts The existing design models the vibration of a single vocal fold under tensile stress and provokes the elongation of fibroblasts and the subsequent formation of an extra cellular matrix characteristic of vocal fold fibroblasts Our goal is to improve upon this model by simulating contact between two vibrating vocal folds and potentially finding a better substitute for Tecoflex because its porosity is non uniform and the pore size is too large The bioreactor will be made from a T flask with elastic vibrators immersed in a buffer which will function as vocal folds This device needs to be easily replaced with disposable parts along with 2 pairs of artificial vocal folds and a capacity to generate computer controlled vibrations at a frequency 0 400Hz Restatement of Team Goals This week s goal is to construct the working prototype with all components attached the actuator vibrating and the Tecoflex strips oriented properly We also need to start working on the poster for the final presentation in the coming week We also need to get the list of expenses and reimburse the team members who have paid for the components individually Individual Goals Dustin Work on the tents and the brass bridge mounting support Karen Send out the progress report restrain actuator make the poster Rachel Richard Work the T flask base get the proper holes drilled cover of the flask work on the bills Summary of Accomplishment z Team meeting on Thursday Apr 26 confirmed that all components have arrived and talked about how to put the parts together We also confirm the working status z Team meeting on Friday Apr 20 worked on the organization and placing of the actuator rods pipettes on the edge of the T flask z Team moved all the parts to advisor s lab for storage We tested out the prototype and found it working There are some room for improvement and adjustment of the prototype z Dustin Karen and Richard met on Monday Apr 30 to put together the flask We drilled holes into the base for the screws that will hold the flask in place Dustin and Richard also drilled holes though the brass bridge that will hold down the actuator rods z Dustin Karen and Richard met on Tuesday May 1 to work on the project Dustin and Richard drilled holes on the base to hold down the brass bridge Karen worked on the poster We also tried to glue some parts together z Dustin Karen and Richard met on Wednesday May 2 for the final wrap up of the prototype Dustin made several tents to prevent the leakage of solution from the flask We also replaced the bandage with the Tecoflex strips Pins were inserted through the actuator rods to prevent translation Dustin got some cloth and barbells for the actuator Karen finished the poster Richard cut out a plastic plate for the raise the height of the flask We took images of the prototype for the poster Statement of Team Goals This week is to produce a good poster and practice for the final poster presentation We need to get the poster printed in places other than College library The last thing to work on is the final report Difficulties We have problems to find a place to print since the printer in the College library has broken down Currently the best printing option is probably Kinko s Activities Dustin Karen 04 28 2007 Bought spare parts 1 50h 04 30 2007 Prototype building 4 00h 05 01 2007 Bought spare parts 1 50h 05 01 2007 Prototype building 3 00h 05 02 2007 Prototype building 3 00h 04 29 2007 Poster layout 0 50h 04 30 2007 Prototype building poster 4 00h 13 00h 13 50h 05 01 2007 Poster 3 00h 05 02 2007 Poster 4 50h 05 02 2007 Prototype building 0 50h 05 03 2007 Progress report 1 00h Rachel Richard 04 30 2007 Prototype building 4 00h 12 50h Team 04 30 2007 Flask drilling bought transparencies 1 50h 05 01 2007 Prototype building 3 00h 05 01 2007 Flask base designing 0 50h 05 02 2007 Balance bills statements 0 50h 05 02 2007 Prototype building 3 00h 04 26 2007 Regular group meeting 1 00h 04 27 2007 Meeting with advisor 0 50h 04 27 2007 Group meeting and moving of the machinery 2 00h 3 50h Project Timeline Jan February Tasks Project research Brainstorming PDS Prototype design Prototype building Actual device design Ordering Expected shipping Device manufacturing Testing Re designing Re testing Meeting with client Group meeting Presentation Progress report Website 26 2 9 16 March 23 2 9 April 16 23 30 6 May 13 20 27 4 9 11 Expenses Statement of Accounts BME Spring 2007 Date Type 16 Mar 2007 16 Mar 2007 17 Mar 2007 17 Mar 2007 17 Mar 2007 18 Mar 2007 10 Apr 2007 12 Apr 2007 21 Apr 2007 21 Apr 2007 22 Apr 2007 22 Apr 2007 27 Apr 2007 27 Apr 2007 27 Apr 2007 29 Apr 2007 30 Apr 2007 30 Apr 2007 30 Apr 2007 Starting Amount Function Generator Power Amplifier Barbell Electromagnetic Voice Coil Actuator Actuator Rods Pipettes Low Mass Vibrational Bar Metal Screws with Plastic Caps Plastic Screws with springs Flat Spring Rollers Hex Bolts Dropcloth 9x 12 5mil Solid Brass Bar 1x 3 64 Paint Brushes Duct Tape Paint Lock Nuts Wing Nuts Washers Metal Short Screws 2 May 2007 2 May 2007 Barbell Grip Cloth Amount Balance 0 256 00 200 00 31 65 711 00 16 14 0 00 4 11 4 22 6 32 1 46 1 36 1 36 2 01 6 32 3 47 4 14 0 44 3 10 4 98 5 000 00 4 744 00 4 544 00 4 512 35 3 801 35 3 785 21 3 785 21 3 781 10 3 776 88 3 770 56 3 769 10 3 767 74 3 766 38 3 764 37 3 758 05 3 754 58 3 750 44 3 750 00 3 746 90 3 741 92 21 10 3 720 82 1 01 3 719 81
View Full Document
Unlocking...