Microscope Manipulator for Zebrafish Analysis Week November 18th 2005 November 27th 2005 Team Members Joe Hippensteel Team Leader Evan Rogers Communications Chris Webster BSAC Jonathan Baran BWIG Client Robert Jeraj Assistant Professor Department of Medical Physics Phone 608 263 8619 Email rjeraj wisc edu Advisor Willis Tompkins Professor Department of Biomedical Engineering Phone 608 263 1581 Email tompkins engr wisc edu Problem Statement Our goal is to develop required devices and techniques for a zebrafish embryo imaging and irradiation research project The initial stage is developing and constructing a working prototype of a digital micromanipulator to move the Petri dish of zebrafish embryos at a reasonable speed and precision to be able to develop a composite image of the entire dish Software is needed to operate the stage The zebrafish must be localized during the initial scan using standard digital imaging techniques This information will be used to irradiate the fish and determine the presence of cell apoptosis and inflammation due to this radiation Last Week s Goals Finish the software fusion Determine maximum operating speed e g finish speed analysis Discuss and decide upon other necessary components radiation blocker camera rig better focus etc Start to plan final report and presentation Accomplishments Finished optimization program and quantified movement Created Solidworks design of irradiator stand Met with clients to discuss progress and necessary deliverables Continued software fusion o Difficulties with image processing o Visual basic portion going smoothly This Week s Goals Finish Construct irradiator stand and check precision Finish code Determine superb set up for client Finish final presentation Finalize final report Difficulties Understanding current code Ridding speed maximization pictures of background noise using Matlab Finding time to meet as a group due to hectic week Activities Accomplishments Group Member Weekly Accomplishments Time hrs Running Total hrs Joe Hippensteel Group meetings micromanipulator Evan Rogers Chris Webster research progress report problem 12 statement revamp code review speed testing analysis Group meeting Solidworks 14 imaging research code review Matlab coding Group meetings imaging research code review speed testing speed 10 testing analysis hardware research 71 70 67 Jonathan Baran BWIG meeting group meetings code review and editing VB research 11 70 5
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