Microscope Manipulator for Zebrafish Analysis Week October 14th 2005 October 20rd 2005 Team Members Joe Hippensteel Team Leader Evan Rogers Communications Chris Webster BSAC Jonathan Baran BWIG Client Robert Jeraj Assistant Professor Department of Medical Physics Phone 608 263 8619 Email rjeraj wisc edu Advisor Willis Tompkins Professor Department of Biomedical Engineering Phone 608 263 1581 Email tompkins engr wisc edu Problem Statement Our goal is to develop required devices and techniques for a zebrafish embryo imaging and irradiation research project The initial stage is developing and constructing a working prototype of a digital micromanipulator to move the Petri dish of zebrafish embryos at a reasonable speed and precision to be able to develop a composite image of the entire dish In addition the zebrafish must be localized during the initial scan using standard digital imaging techniques This information will be used to irradiate the fish and determine the presence of cell apoptosis and inflammation due to this radiation Last Week s Goals Begin fusion of disparate programs Determine most cost efficient method for stage manipulation Commercial or custom Accomplishments Began software fusion Determined some possibilities of parts to use This Week s Goals Meet with client to discuss progress Understand direction of project Difficulties Opening Visual Basic program Seems to be a file missing Activities Accomplishments Group Member Weekly Accomplishments Time hrs Running Total hrs 5 37 5 Group meeting client meeting imaging research 5 37 Group meeting class time client meeting imaging research 5 37 5 5 39 Joe Hippensteel Group meeting class time client meeting micromanipulator research Evan Rogers Chris Webster Jonathan Baran group meeting class time client meeting
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