Guided Reading Chapter 10 Read pages 182 189 1 In your own words what is molecular biology 2 How did Fredrick Griffith s work demonstrate that cells had a transforming factor Griffith s work set the stage for the identification of the transforming factor but it wasn t until later that Hershey and Chase figured out that this factor was DNA Lets think about how they did this 3 What is a T2 phage and what did Hershey and Chase know about the T2 phage prior to conducting their experiments 4 What about their experimental design allowed Hershey and Chase to differentiate between DNA and protein 5 How did Hershey and Chase label the DNA and the protein of T2 6 Without looking at your book try to label the figure above If you don t look at your book and you can label this figure appropriately and describe why you labeled things the way you did you likely understand the experiment 7 What was the significance of the blender in the Hershey and Chase experiments 8 What was the significance of the centrifuge in the Hershey and Chase experiments 9 What did Hershey and Chase measure at the end of their experiment and how did their result show that DNA is the transforming factor 10 What are the 3 main components of nucleotides 11 Although the physical attributes and chemical bonding of DNA were known the structure of DNA remained a mystery Rosalind Franklin s x ray image of DNA suggested that the diameter of a DNA molecule was uniform This suggested that DNA was a double helix What did this data suggest about base pairing 12 What does it mean to be complementary as a base pair 13 There are hydrogen bonds between adenine and thymine while cytosine and guanine base pairs have hydrogen bonds For this reason base pairs are weaker than base pairs 14 Each gene has a unique base sequence Why 15 Explain why the sequence of bases differs between genes
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