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TAMU BIOL 111 - Lab Exam 1 Review
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Lab Exam 1 Review 1. EXPERIMENTATION ANDEXPERIMENTAL DESIGN:a. Hypothesis- tentative explanation.i. Null hypothesis- “no effect” from independent variable.ii. Experimental or alternative hypothesis- “will effect” from independent variable.b. Independent variable-variable you manipulate from on experimental group to the next.c. Dependent variable- the variable you measure.d. Control treatment group- the treatment group not subject to the independent variable.e. Multiple constants- anything kept the same from one treatment group to the next.f. Experimental Treatment group- the treatment groups subject to the independent variable.g. Interpret data to make an inference or draw a conclusion.2. METRIC MEASUREMENT AND CALCULATION OF DENSITY:a. Always use metric***b. Always put wiggle digit***c. Mass (grams)d. Volume (cm3 or mL)- 2 decimals for eachi. L x W x H= cm3ii. Irregular objects--Displacement: Final volume - Initial volume = mLe. Density (g/mL or g/cm3)-i. Mass/Volume = Densityf. Metric scale chart (pg 52)g.3. MOLAR SOLUTIONS: a. Preparing A stock solution = (required volume in L) x (required molarity in M) x (mol. Wt.of compound)b. Ex: (3 liters) x (0.5 M) x (95.21g/mole of MgCl2) = 142.82g of MgCl2 is needed c.4. BIOCHEMICAL TESTS USING REAGENTS:a. Sugar (Reducing sugar)- Simple Carbohydrates: Benedict’s reagent, heat, positive color change is green to dark orange.b. Starch (polysaccharide)- Complex Carbohydrate: Lugol’s Iodine, mix, positive color change is dark blue/black.c. Protein- Biuret reagent, mix, positive color change is violet.d. Lipids- paper strip, opaque=neg. translucent stain clear=pos.e. When stating results, say what is present and not present. 5. MICROSCOPE CELL RECOGNITION AND DOCUMENTATION: pg 106-107a.b. Label two or more features/structures and properly document your specimen using proper drawings.i. LOOK AT PAGES 106-108ii. Bacteria:1. Shape-a. Cocci- sphericalb. Bacilli- rodsc. Spirilla- spirals2. Arrangement-a. Diplo- pairsb. Strepto- chainsc. Staphylo- clustersc. Calculate total magnification.i. Total = (Obj.) x (Ocular)d. Identify specimen.e. State what type of cell- Eukaryote or Prokaryote?f. Identify domain.i. Eukaryotes- Eukarya- larger w/nuclei and membranous organellesii. Prokaryotes- Bacteria or Archaea- lack nucleus and lack membranous organelles.6. OSMOLARITY AND GRAPHING:a.b. % change in mass = ((final-intial)/intial) x


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TAMU BIOL 111 - Lab Exam 1 Review

Type: Study Guide
Pages: 3
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