Berkeley ELENG C235 - Label-Free Protein Biosensor Based on Aptamer-Modified Carbon Nanotu be Field-Effect Transistors - D2006535

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Berkeley ELENG C235 - Label-Free Protein Biosensor Based on Aptamer-Modified Carbon Nanotu be Field-Effect Transistors

School name University of California, Berkeley

Course Eleng C235- Nanoscale Fabrication

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Slide 1Slide 2Slide 3Slide 4Slide 5Slide 6Slide 7Slide 8Label-Free Protein Biosensor Based on Aptamer-Modified Carbon Nanotube Field-Effect TransistorsKenzo Maehashi, Taiji Katsura, Kagan Kerman, Yuzuru Takamura, Kazuhiko Matsumoto, and Eiichi TamiyaAnal. Chem. 2007, 79, 782-787Jui-Ming (Ryan) YangRyan Yang / 03.05.2007OutlineBackgroundCNT applicationsCNT biosensor : detection of Immunoglobulin E Experimental SetupResultConclusion / ConsiderationRyan Yang / 03.05.2007BackgroundCNT – FETCNT – Gas SensorCNT – BiosensorProtein SensorDNA SensorLin, Y.-M., et al., IEEE Electron Device Lett. (2005) 26, 823S Meng, et al. Nano Letter (2007) 7 1 45-50A Modi et al. (2003) Nature 424 171Ryan Yang / 03.05.2007BackgroundGoal: Detection of Immunoglobulin E (IgE)Aptamer (synthetic oligonucleotides)Monoclonal Antibody against IgE (IgE-mAb)Debye Length: distance required for screening the surplus charge by mobile carriers present in the materialRyan Yang / 03.05.2007Experimental SetupCNT-FET p+ type substrate / 100nm SiO2source – drain contact: Ti/Auethanol thermal CVD growth CNTCustom-Synthesized DNA AptamerImmobilized Aptamer onto CNT1-pyrenebutanoic acid succinimidyl esterwith dry dimethylformamide solutionsexpose to aptamer in PBS overnightdeactivate and block the excessreactive groups via ethanolamineD17.4ext: 5’-NH2-GCG CGG GGC ACG TTT ATC CGTCCC TCC TAG TGG CGT GCC CCG CGC-3’Ryan Yang / 03.05.2007Resultp-type characteristics in blank PBSThe current increased after the IgE-aptamer modification (at the source-drain bias of 0.2 V )Stability testSelectivity test Brovie serum albumin (BSA) binding eventRyan Yang / 03.05.2007ResultSharp decrease in the source-drain current after introducing IgE, then saturation at lower valuesNon-specific binding of IgE can be suppressedComparison to IgE-mAb modified CNT-FETRyan Yang / 03.05.2007ConclusionAptamer modified CNT detects the immunoglobulin E with a good dynamic range, from 250pM to 20 nMPromising candidate for the development of an integrated, high-throughput, real-time biosensorConsiderationExperimental setup scheme: gate referenceSignal target detection

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Berkeley ELENG C235 - Label-Free Protein Biosensor Based on Aptamer-Modified Carbon Nanotu be Field-Effect Transistors

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