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Recitation 8 DNA Replication Central dogma DNA RNA Protein Semi conservative replication Direction 5 to 3 Enzymes involved in DNA replication Unzipping the two strands helicase Okazaki fragments Assembling the nucleaotides DNA polymerace Joins together DNA fragments ligase Repair mechanism activity that DNA polymerase has exonuclease Mismatch repair endonuclease Transcription Translation Process of making RNA from DNA RNA is single stranded while DNA is double stranded RNA has a base U while DNA has a base T RNA has a 2 OH while DNA is deoxy Direction 5 to 3 There are promoters that tells the enzymes which strand to use as the template Catalyzes transcription RNA polymerase Able to initiate by itself Knows to stop when it gets to the stop signal termination signal Process of making protein from RNA Template mRNA Ribosome catalyzes the reaction Knows where to start from the start codon Methenamine is the start codon Direction 5 to 3 N NH3 terminal to C COO terminal No amino acids are inserted at the stop codon There are three stop codons


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MIT 7 012 - Recitation 8

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