MIT 7 012 - Study Guide (7 pages)

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Study Guide



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Study Guide

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Pages:
7
School:
Massachusetts Institute of Technology
Course:
7 012 - Introduction to Biology
Introduction to Biology Documents

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MIT Biology Department 7 012 Introductory Biology Fall 2004 Instructors Professor Eric Lander Professor Robert A Weinberg Dr Claudette Gardel Solutions to 7 012 Problem Set 8 Question 1 You have isolated cells from an alien organism called Sacul Sinned You want to know if these cells contain proteins that are similar to the cyclins found in eukaryotic cells To this end you radioactively label all the proteins in the cell to allow for visualization on a gel You then synchronize the cells by placing them in low serum for 1 day Finally you stimulate the cells with PDGF and isolate protein from the cells at different time points The proteins are then run out on a gel similar to a DNA gel such that the proteins are separated by size and you can visualize them through their radioactivity 1 2 3 4 5 6 7 8 a Circle the protein bands on the gel that would be good candidates for cyclin proteins Explain your choices Any of the bands that appear and then disappear during your time course would be good choices as potential cyclin proteins because cyclin proteins are only present during the part of the cell cycle that they regulate b How many cell cycles did your time points cover if each of the protein bands you selected above are actually cyclin proteins Explain you answer The time points cover 2 cell cycles Cyclins are proteins that bind to cyclin dependant kinases and activate them The cyclin proteins are only present during the part of the cell cycle that they regulate If the bands that you circled are cyclin proteins then the fact that each of them appears twice over your time course suggests that your cells have gone through two cell divisions 7 012 Fall 2003 1 Question 1 continued c What does synchronization do to the cells and why is it necessary for this experiment Synchronization places all the cells in the same stage of the cell cycle such that as the cell cycle progresses they enter the other phases at the same time It is necessary for the above experiment because if



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