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Why did he heat the virulent bacteria It kills the bacteria because it denatures the protein Transformation element caused the heated virulent and the non virulent to be deadly DNA is the transforming principal Recitation 7 Grif th studied pneumecocus Virulent deadly Non virulent harmless Three parts of DNA Triphosphate 2 deoxyribose Nitrogenous base Purines A G Pyrimidines C T Hershey and Chase Bacteriophage made up of only DNA and protein viruses that infect bacteria and tag proteins vs DNA Proteins have sulfer cystein and metheine and DNA has phosporous S and P are tagged They tried to nd whether protein or DNA was being inserted into the bacteria They put the bacteria into the blender and then the centrifuge They found P on the bottom showing that DNA was inserted into the bacteria DNA structure Chargaff s Rule A T and C G Double helix DNA going 5 to 3 and the other one going from 3 to 5 because they are antiparallel The sugar phosphate backbone is on the outside Replication Transcription DNA RNA Translation RNA Protein Messeisn and Stahl Used radiocative N and N They then used a centrifuge to see where the nitrogen would settle based on weight Semi conservative model in the nal produce one strand will be new and one will be the original template Conservative model strands stay together Dispersive model the strands will nave both new and old components to it


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MIT 7 012 - Recitation

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