One of the best known models of enzyme kineticsIntrinsic catalytic properties of the enzymes Km substrate that enzyme works best at If Keq is greater than one there are more products If Keq is very large we consider the reaction irreversible If Keq is less than one there are more reactants If Keq is very small we consider the reaction impossible Recitation Enthalpy thermodynamic potential H Entropy disorder S Temperature measured in kelvin T Michaelis Menten kinetics denotes concentration Equilibrium Keq High Energy Molecules ATP NADH FADH Electron Transport Chain ETC Produces 38 APT requires oxygen Fermentation Regenerates NAD Recitation questions Would you expect the overall G of glucose pyruvate to be positive or negative The overall G is negative because high energy molecules are being produced How many different enzymes do you expect are involved in glycolysis 10 enzymes because there are 10 steps In what types of organisms would you expect to nd these enzymes Which of the steps in glycolysis would have an energy pro le with a shape like that shown in the handout In the absence of oxygen fermentation occurs instead of respiration Steps 5 6
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