Analysis 7 1 The PCR reactions did 40 rounds of amplification If there was only a single DNA molecule in the starting reaction how many were there when it ended 240 DNA molecules were left at the end of the PCR reactions 2 The average weight of a DNA basepair bp either AT or GC is approximately 650 g mole If the average size of the amplified region is 300 bp How much does the amplified DNA weigh Show your work Amplified DNA 300 bp Molecular weight 1 mol of DNA bp 6 02x1023 base pairs Weight 1 mol bp 650 g 650 g 6 02x1023 bp 1 08x10 21 g per bp 300 bp X 1 08x10 21 g bp 3 24 x 10 19 g 3 Starting with the original DNA template draw four rounds of PCR products Include and label the primers oligos All strands of DNA and primers oligo must include a 5 and 3 label Make it clear which stands were newly synthesized in each round and indicate the direction of synthesis using an arrow Data Analysis 4 How many different genotypes patterns do you see for each set of oligos primers For master mix 1 M1 the gel electrophoresis results show clear bands indicating successful amplification of DNA and the presence of discernible patterns which is 2 or 3 However because master mixes 2 and 3 M2 and M3 did not produce any visible bands it is impossible to determine different genotypes for those samples This inconsistency in results limits the ability to compare or count different genotypes across all sets of oligos primers Therefore for M1 we may see multiple patterns but for M2 and M3 we are unable to observe or identify any genotype patterns due to the absence of amplified DNA 5 Are there are bees that have the same genotype at all 3 loci tested For master mix 1 M1 the gel electrophoresis results show clear bands indicating successful amplification of DNA and the presence of discernible patterns but they are not uniform However because master mixes 2 and 3 M2 and M3 did not produce any visible bands it is impossible to determine different genotypes for those samples This inconsistency in results limits the ability to compare or count different genotypes across all sets of oligos primers Therefore for M1 we may see multiple patterns but for M2 and M3 we are unable to observe or identify any genotype patterns due to the absence of amplified DNA 6 Can you tell how many drones males mated with the queen female that was the mother of all these bees Is there evidence for more than one Is there a genotype sample from a bee that would make this easier to determine In the M1 sample alone there are distinct banding patterns indicating that multiple genotypes are present among the bees tested If the queen had mated with only one drone it would be expected to see a more uniform pattern across all samples as the offspring would inherit the same alleles from both parents However the presence of multiple banding patterns in the M1 sample suggests that the queen likely mated with multiple drones each contributing unique alleles
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