Central Dogma of Molecular Biology DNA Replication Notes for 4 6 15 BSCI105 Lecture I Chromosome Structure a Prokaryotes i One chromosome ii Circular iii 5 million base pairs ex E Coli b Eukaryotes i Many chromosomes ii Linear iii 6000 million base pairs ex Humans c DNA replication i Watson Crick model of DNA suggested a mechanism for replication 1 Parental molecule 2 Separation of strands unzipping 3 Daughter DNA molecules with one parental strand and one new strand ii Semi conservative replication 1 One parent strand and one daughter strand 2 New double helix hybrid of old and new II Mechanism of DNA replication a CLICKER QUESTION which of the following tasks is NOT required for DNA replication and is not an active process rewinding the DNA b What do you need i Enzymes ii Enzymes needed for bacterial DNA replication 1 Helicase 2 Single strand binding protein 3 Topoisomerase 4 Primase 5 DNA pol 3 6 DNA pol 1 7 DNA ligase c What do you do i Separate strands ii Pick a starting point iii Make new DNA strands d Where do you start i Origin of replication 1 One in prokaryotes 2 Many in eukaryotes e Task 1 separate strands i Enzyme topoisomerase 1 Cuts 1 strand of double helix 2 Relieves strain when unwinding ii Helicase 1 Unwinds DNA
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