Bacillus cereus Food poisoning Large rods spores Borrelia borgdurferc Lyme disease transmitted by tick Spirals Two drops of Hydrogen peroxide on slide add bacteria If bubbling breakdown of hydrogen peroxide to water O2 Can it break down gelatin Tests for gelatinase If liquefaction Can it break down tryptophan Add ten drops kovak s reagent Tests for the presence of If red layer Catalase Gelatin Indole Methyl Red Mycobacterium tuberculosis Nitrate Broth Oxidase Measures production of acids Add five drops If red Yellow Tuberculosis lung disease Small rods neither or If gas bubble denitrification Add three drops Nitrate A 3 Nitrate B If red at top nitrate reduced to nitrite Bacteria on swab add If purple Pseudomonas aeruginosa Small rods burn and wound infections SIM agar Simons Citrate Agar Starch agar Sugar fermentation Tests for hydrogen sulfide production If blackening along stab line for H2S Indicates use of citrate as carbon source development of alkaline PH If turns blue Add iodine If halo Indicates hydrolysis breakdown of Yellow indicates acid fermentation If bubble produces gas Separate tests for glucose sucrose lactose and mannitol
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