BIO 446L 1st Edition Lecture 1Outline of Last Lecture I. n/aOutline of Current Lecture I. Histology Methods a. Staini. H and E and PASb. Fluorescence and immunofluorescencec. Phase contrast and differential interfaced. Polarized lighte. Confocal microscopyf. Immunohistochemistryg. Electron microscopyi. TEM and SEMCurrent LectureHistology Methods- Histology= Focuses on how cells structure and arrangement optimize functions specific to each organ- Staino H & E Can see cells but not clearly where one begins and one ends- Ex: can see nuclei, goblet cells, etc. Can see shape but not much detailo PAS Cells stain darker- Stains very dark where there are lots of carbohydratesThese notes represent a detailed interpretation of the professor’s lecture. GradeBuddy is best used as a supplement to your own notes, not as a substitute. Can see more distinctly, for example, the wall of epithelial cells that line the lumen More contrast but also not much detail- Fluorescence and immunofluorescenceo Using proteins/antibodies that bind specifically to a cell component and fluorescewhen excited with light Ex: can see nucleic acids and even differ from DNA and RNA by fluorescing in different colors- Phase Contrast and differential interferenceo Phase contrast Can see much more detail, structures, processeso Differential Makes structure 3D looking - Polarized lighto Good for viewing very organized structures- Confocal microscopyo Laser light, using phase contrast (see slide pictures for examples)- Immunohistochemistryo Can identify protein of interest- Electron microscopyo TEM and SEM See slide images for examples o
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