Two loci long chrom long chrom short chrom Parent A short chrom AA AA x BB BB A A Parent B A A B B locus 2 locus 1 B B locus 2 AB AB F1 Locus1 Locus 2 AB AB x AB AB 16 possibilities F2 locus 1 Two loci incomplete dominance new and improved QuickTime and a TIFF Uncompressed decompressor are needed to see this picture 2 locus interaction For example locus 2 is polymorphism in a transcription factor gene that affects protein binding affinity locus 1 is in a binding site QuickTime and a TIFF Uncompressed decompressor are needed to see this picture Effect of J at locus 2 Effect of variation at locus 2 depends on genotype at locus 1 non additive NO progeny as extreme as diploid hybrid QuickTime and a TIFF Uncompressed decompressor are needed to see this picture NO progeny as extreme as diploid hybrid Lab parent Pathogenic parent Diploid hybrid Lab Pathogenic Different in sequence Lab Pathogenic Tested in hybrid diploids One mutant gene From pathogenic strain QuickTime and a TIFF Uncompressed decompressor are needed to see this picture QuickTime and a TIFF Uncompressed decompressor are needed to see this picture One mutant gene From pathogenic strain QuickTime and a FromTIFF lab strain Uncompressed decompressor are needed to see this picture QuickTime and a TIFF Uncompressed decompressor are needed to see this picture Two mutant genes From pathogenic strain QuickTime and a From lab strain TIFF Uncompressed decompressor are needed to see this picture QuickTime and a TIFF Uncompressed decompressor are needed to see this picture Two mutant genes From pathogenic strain QuickTime and a From lab strain TIFF Uncompressed decompressor are needed to see this picture QuickTime and a TIFF Uncompressed decompressor are needed to see this picture Two mutant genes From pathogenic strain QuickTime and a From lab strain TIFF Uncompressed decompressor are needed to see this picture Again diploid with this gene from the pathogenic strain grows BETTER at high T Three mutant genes From lab strain From pathogenic strain QuickTime and a TIFF Uncompressed decompressor are needed to see this picture Diploid with this gene from the pathogenic strain grows WORSE at high T Three mutant genes From pathogenic strain From pathogenic strain From pathogenic strain QuickTime and a TIFF Uncompressed decompressor are needed to see this picture Three mutant genes From pathogenic strain From pathogenic strain From pathogenic strain QuickTime and a TIFF Uncompressed decompressor are needed to see this picture QuickTime and a TIFF Uncompressed decompressor are needed to see this picture Three mutant genes From pathogenic strain From pathogenic strain From pathogenic strain Alleles from the same strain at different genes loci can have different effects QuickTime and a TIFF Uncompressed decompressor are needed to see this picture Three mutant genes in same locus QuickTime and a TIFF Uncompressed decompressor QuickTime and a TIFF Uncompressed decompressor are needed to see this picture are needed to see this picture Three mutant genes in same locus QuickTime and a TIFF Uncompressed decompressor QuickTime and a TIFF Uncompressed decompressor are needed to see this picture are needed to see this picture Three mutant genes So why did 0 900 haploid progeny have a phenotype as extreme as the diploid hybrid NO progeny as extreme as diploid hybrid Lab parent Pathogenic parent Diploid hybrid NO progeny as extreme as diploid hybrid QuickTime and a QuickTime and a TIFF Uncompressed decompressor TIFF Uncompressed decompressor are needed to see this picture are needed to see this picture Lab parent Pathogenic parent Diploid hybrid Linked mutations of opposite effect Path Linked mutations of opposite effect Path Lab Linked mutations of opposite effect Path Lab Very unlikely Multiple linked loci Model organism to human Why pigmentation Camouflage Sexual selection Protection from UV Controlling light scatter Sunlight for vitamin D Accident something else selected for nearby Golden mutation QuickTime and a TIFF Uncompressed decompressor are needed to see this picture Golden mutation QuickTime and a TIFF Uncompressed decompressor are needed to see this picture Melanosomes melanin producing organelles Golden mutation 1981 original mutant arose spontaneously in the lab Showed mendelian segregation 1 locus and is recessive Golden mutation 1981 original mutant arose spontaneously in the lab Showed mendelian segregation 1 locus and is recessive Dramatic single locus differences not common in the wild Why not Linkage analysis with sparse markers mapped golden to markers on chromosome 18 but highresolution position of markers unknown Linkage analysis with sparse markers mapped golden to markers on chromosome 18 but highresolution position of markers unknown Genome sequence not completed Linkage analysis with sparse markers mapped golden to markers on chromosome 18 but highresolution position of markers unknown Genome sequence not completed Hybrid cell lines not available Library screen Shear ligate Native WT chromosomes Library screen Shear ligate Native WT chromosomes Genomic library Library screen Shear ligate Native WT chromosomes Genomic library Hybridize to marker oligo 85 kb DNA containing chr 18 marker Fine mapping 85 kb Inject into golden larvae Fine mapping 85 kb QuickTime and a TIFF Uncompressed decompressor are needed to see this picture Inject into golden larvae Fine mapping Golden uninjected 85 kb QuickTime and a TIFF Uncompressed decompressor are needed to see this picture Inject into golden larvae Fine mapping WT uninjected Golden uninjected 85 kb QuickTime and a TIFF Uncompressed decompressor are needed to see this picture Inject into golden larvae Fine mapping WT uninjected Golden uninjected 85 kb QuickTime and a TIFF Uncompressed decompressor are needed to see this picture Inject into golden larvae mosaic rescue Fine mapping 85 kb Fine mapping 85 kb slc24a5 mRNA Fine mapping 85 kb QuickTime and a TIFF Uncompressed decompressor are needed to see this picture slc24a5 mRNA Inject into golden larvae Fine mapping WT uninjected Golden uninjected QuickTime and a TIFF Uncompressed decompressor are needed to see this picture QuickTime and a TIFF Uncompressed decompressor are needed to see this picture Inject into golden larvae Gene is strongly conserved Looks like a Na Ca exchange pump Gene is strongly conserved Looks like a Na Ca exchange pump Gene is strongly conserved Looks like a Na Ca exchange pump premature stop codon in DNA of golden
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