Making linkage analysis simpleSlide 2Bakers’ yeastHaploid and diploidSlide 5Slide 6Slide 7Why yeast?Tetrad analysisSlide 10Slide 11Slide 12Slide 13Slide 14Slide 15Slide 16Slide 17Slide 18Slide 19Tetrad analysis exampleSlide 21Slide 22Slide 23Tetrad analysis example 2Slide 25Slide 26Slide 27Accurate genetic distances?The real story: mapping functionsBread moldSlide 31Slide 32Mapping centromeric distanceSlide 34Slide 35Making linkage analysis simpleMaking linkage analysis simple(Do Ch. 5 problems!)Bakers’ yeastA single-celled eukaryoteHaploid and diploidFig. 5.14Haploid and diploidFig. 5.14Clonal (mitotic) growthHaploid and diploidFig. 5.14Haploid and diploidFig. 5.14Why yeast?Tetrad analysisFig 5.15Tetrad analysisFig 5.15Tetrad analysisFig 5.15Cannot make histidineCannot make tryptophanTetrad analysisFig 5.15Tetrad analysisFig 5.15Tetrad analysisFig 5.15Tetrad analysisFig 5.15Tetrad analysisFig 5.15Tetrad analysisNPD = 4 recombinant progenyFig 5.15Tetrad analysisFig 5.15Tetrad analysisFig 5.15T = 2 recomb, 2 non-recombTetrad analysis exampleFig 5.15Tetrad analysis example“Number of recombinants = NPD•4 + T•2”Fig 5.15Tetrad analysis example“Number of recombinants = NPD•4 + T•2”“RF = NPD•4 + T•2 ”4•Total # tetradsFig 5.15Tetrad analysis example“Number of recombinants = NPD•4 + T•2”“RF = NPD•4 + T•2 ”Total # tetrads4•Total # tetrads= NPD + 1/2T ” Fig 5.15Tetrad analysis example 2Fig. 5.16Tetrad analysis example 2If PD >> NPD, genes are linked.Fig. 5.16Tetrad analysisFig 5.15If linked, second law does not hold;<< 50% recombinantsTetrad analysisWhat if two crossovers?Fig 5.15Accurate genetic distances?“Number of recombinants = NPD•4 + T•2”“RF = NPD•4 + T•2 ”Total # tetrads4•Total # tetrads= NPD + 1/2T ” Where’s PD?The real story: mapping functionsFig. 5.17Do Ch. 5, problem 35…Bread moldQuickTime™ and aTIFF (Uncompressed) decompressorare needed to see this picture.Bread moldFig. 5.14Bread moldFig. 5.14Each pair is identicalMapping centromeric distanceFig. 5.21Mapping centromeric distanceFig. 5.21Mapping centromeric distanceFig. 5.21Number of meioses with second-division patterns = number of crossovers between locus and
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