Making linkage analysis simple Making linkage analysis simple Do Ch 5 problems Bakers yeast A single celled eukaryote Haploid and diploid Fig 5 14 Haploid and diploid Clonal mitotic growth Fig 5 14 Haploid and diploid Fig 5 14 Haploid and diploid Fig 5 14 Why yeast Tetrad analysis Fig 5 15 Tetrad analysis Fig 5 15 Tetrad analysis Cannot make histidine Fig 5 15 Cannot make tryptophan Tetrad analysis Fig 5 15 Tetrad analysis Fig 5 15 Tetrad analysis Fig 5 15 Tetrad analysis Fig 5 15 Tetrad analysis Fig 5 15 Tetrad analysis Fig 5 15 NPD 4 recombinant progeny Tetrad analysis Fig 5 15 Tetrad analysis Fig 5 15 T 2 recomb 2 non recomb Tetrad analysis example Fig 5 15 Tetrad analysis example Number of recombinants NPD 4 T 2 Fig 5 15 Tetrad analysis example Number of recombinants NPD 4 T 2 RF Fig 5 15 NPD 4 T 2 4 Total tetrads Tetrad analysis example Number of recombinants NPD 4 T 2 RF Fig 5 15 NPD 4 T 2 4 Total tetrads NPD 1 2T Total tetrads Tetrad analysis example 2 Fig 5 16 Tetrad analysis example 2 Fig 5 16 If PD NPD genes are linked Tetrad analysis Fig 5 15 If linked second law does not hold 50 recombinants Tetrad analysis What if two crossovers Fig 5 15 Accurate genetic distances Number of recombinants NPD 4 T 2 RF NPD 4 T 2 4 Total tetrads NPD 1 2T Total tetrads Where s PD The real story mapping functions Fig 5 17 Do Ch 5 problem 35 Bread mold QuickTime and a TIFF Uncompressed decompressor are needed to see this picture Bread mold Fig 5 14 Bread mold Each pair is identical Fig 5 14 Mapping centromeric distance Fig 5 21 Mapping centromeric distance Fig 5 21 Mapping centromeric distance Fig 5 21 Number of meioses with second division patterns number of crossovers between locus and centromere
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