Mosaic Analysis Reading Chapter 5 pp140 141 Reference chapter D pp820 823 Problem set F Twin spots in Drosophila Although segregation and recombination in mitosis do not occur at the same frequency as in meiosis under the proper conditions crossing over can be observed in mitosis Curt Stern was the first to describe mitotic recombination In females heterozygous for the recessive X linked yellow body y and the singed bristle sn alleles y sn y sn Stern noted that most females are wild type with grey bodies and normal bristles However some females had patches of yellow or singed short twisted bristles Other females had twin spots or two adjacent regions one exhibiting a yellow phenotype and the other exhibiting a singed phenotype Since the two spots were adjacent Stern reasoned that the twin spots were reciprocal products of the same event the product of mitotic crossing over Since the order on the X chromosome is centromere sn y a crossover between the centromere and the sn gene will result in a twin spot A single singed patch can be explained by mitotic nondisjunction and a yellow patch can be explained by mitotic nondisjunction or by a mitotic crossover between the sn and y genes Individuals that are composed of cells of more than one genotype are referred to as genetic mosaics The use of genetic mosaics to determine the site of gene function It turns out that the Sevenless protein is expressed in many cells of the developing ommatidium How then can we determine in which cells sevenless functions Drosophila geneticists can use mosaic animals to address this question Let s consider the experiments to determine the site of Sevenless function first and then we will turn to Boss The basic approach is to make mosaic animals that have some cells in the eye that are functionally wild type for Sevenless and others that are mutant Figure 1 X rays stimulate mitotic recombination causing frequent crossing over between homologs during mitosis it is thought that induced chromosomal breaks stimulate mitotic recombination When mitotic recombination is stimulated in animals that are heterozygous for the sev mutation clones of homozygous mutant cells are produced We also need to know which cells are mutant and the chromosome bearing the sev mutation also contains a white mutation Mosaic eyes will have patches of white tissue These eyes are sectioned and the cells that are wild type for white and hence wild type for sev contain pigment granules while cells that are mutant for white and hence mutant for sev don t contain pigment granules The white gene is necessary for the transport of pigment granules Figure 1 X ray induction of a mitotic recombination event that will lead to a mosaic clone Note that the alternate orientation of the homologous chromosomes would result in a segregation pattern that would not lead to mosaic clones In the mosaic eyes some ommatidia will consist of mixtures of wild type and mutant cells There were no ommatidia in which the R7 cell was white Moreover as long as the R7 cell was wild type for white any or all of the cells could be mutant Figure 2 These results indicate that the requirement for the wild type sev gene product is in the R7 cell and we say that the requirement for sev is cell autonomous In other words to develop into an R7 cell and not a cone cell a presumptive R7 cell must make the Sev protein and it doesn t matter whether other cells of the same ommatidium make Sev protein Figure 2 Examples of sev mosaic ommatidia where the R7 cell is produced Only the photoreceptors cells are shown in the diagram 1 is R1 2 is R2 etc When researchers performed mosaic analysis for the boss gene the results were different An R7 cell develops properly only if the R8 cell is wild type for boss All of the other cells of the can be mutant for boss including R7 and R7 will develop normally as long as R8 is wild type Conversely if R8 is mutant even when all of the other cells of the ommatidium are wild type the presumptive R7 cell will develop into a cone cell These results indicated that the wild type boss gene functioned in the R8 cell to specify the R7 fate and we say that the requirement for the gene is cell nonautonomous This is consistent with the view that Boss is the Sev ligand Figure 3 Examples of ommatidia mosaic for boss Note that there is a correlation between R8 being wild type for boss and the presence of R7 Problem set F 1 Although the mechanism for generating mosaic animals in C elegans and Drosophila is different the principle is the same a mosaic animal is generated and a cell autonomous marker like white in the fly eye is used to determine which cells are wild type and which cells are mutant for the gene being studied Acetylcholine is the neurotransmitter that is released from motor neurons in C elegans and stimulates muscles that control movement to contract by binding to acetylcholine receptors on the muscle cells Acetylcholinesterase is the enzyme that breaks down acetylcholine in the synapse to ensure that the neurotransmitter acetylcholine does not accumulate ace 1 is the gene that encodes the acetylcholinesterase enzyme and recessive mutations in the gene lead to animals that move abnormally This abnormal or uncoordinated movement is caused by the buildup of acetylcholine in the neuromuscular synapse You are interested in determining whether ace 1 gene function is required in motor neurons in muscles or in both motor neurons and muscles You generate mosaic animals that have the recessive ncl 1 mutation and the ace 1 mutation linked so that when cells loose the wild type ncl 1 gene they also loose the wild type ace 1 gene The loss of wild type ncl 1 function is cell autonomous and leads to cells with large nucleoli Thus ncl 1 can be used as a cell autonomous marker in this strain for the loss of ace 1 in specific cells The division of the C elegans zygote produces the AB and P1 cells All of the neurons in C elegans are derived from the AB cell and all of the muscles are derived from the P1 cell Describe the results from your mosaic analysis if ace 1 acetylcholinesterase is produced by motor neurons by muscle by both neurons and muscles Be sure to include which cells contain normal nucleoli Ncl and which cells contain enlarged nucleoli Ncl in the different mosaic animals and which animals are uncoordinated Unc 2 In a screen you identify several new recessive mutations that lead to a loss of R7 You are interested in defining where the genes function and
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