BIOL 1107 1nd Edition Lecture 20Outline of Last Lecture I. DNA EvidenceII. DNA in CellIII. Amplifying DNA with PCR (Polymerase Chain Reaction)IV. History (Rosalind Franklin & Watson + Crick)V. Components of DNAVI. How is DNA Copied?Outline of Current Lecture I. DNA ReplicationII. Replication ForkIII. Chromosome endsCurrent LectureI. DNA Replication- Process of producing two identical replicates from one original DNA molecule. This biological process occurs in all living organisms and is the basis for biological inheritance. These notes represent a detailed interpretation of the professor’s lecture. GradeBuddy is best used as a supplement to your own notes, not as a substitute.II. Replication Fork - Site on a DNA molecule at which unwinding of the helices and synthesis of daughter molecules are both occurring. - RNA primer added to template DNA strand to initiate transcription of RNA- Okazaki fragments- short, newly synthesized DNA fragments that are formed on the lagging template strand during DNA replication- Ends of DNA strands -> problem? Shorter and shorter daughter molecules III. Chromosome ends- Telomeres- natural end of a eukaryotic chromosome composed of a usually repetitive DNA sequence and serving to stabilize the chromosome- Telomerase- an enzyme that adds nucleotides to telomeres, especially in cancer cells.o Telomerase adds repeats to the ends – keep from shorteningQ: PCR us targeted DBA replication in a tube. Below are some of the enzymes involved in DNA replication. Which of these are not used during a PCR reaction?A: Used- DNA polymerase Not used- DNA ligase, helicase, primase, topoisomerase Q: You need to set up a PCR reaction. List all of the ingredients you need to include in the test tube in order for replication to take place. A: DNA polymerase, primers (own own), template, nucleotides- Automated gels for DNA analysis- Short tandem repeats (STR) analysis- a molecular biology method used to compare specific loci on DNA from two or more
View Full Document
Unlocking...