BIOL 1107 1nd Edition Lecture 20 Outline of Last Lecture I DNA Evidence II DNA in Cell III Amplifying DNA with PCR Polymerase Chain Reaction IV History Rosalind Franklin Watson Crick V Components of DNA VI How is DNA Copied Outline of Current Lecture I DNA Replication II Replication Fork III Chromosome ends Current Lecture I DNA Replication Process of producing two identical replicates from one original DNA molecule This biological process occurs in all living organisms and is the basis for biological inheritance These notes represent a detailed interpretation of the professor s lecture GradeBuddy is best used as a supplement to your own notes not as a substitute II Replication Fork Site on a DNA molecule at which unwinding of the helices and synthesis of daughter molecules are both occurring RNA primer added to template DNA strand to initiate transcription of RNA III Okazaki fragments short newly synthesized DNA fragments that are formed on the lagging template strand during DNA replication Ends of DNA strands problem Shorter and shorter daughter molecules Chromosome ends Telomeres natural end of a eukaryotic chromosome composed of a usually repetitive DNA sequence and serving to stabilize the chromosome Telomerase an enzyme that adds nucleotides to telomeres especially in cancer cells o Telomerase adds repeats to the ends keep from shortening Q PCR us targeted DBA replication in a tube Below are some of the enzymes involved in DNA replication Which of these are not used during a PCR reaction A Used DNA polymerase Not used DNA ligase helicase primase topoisomerase Q You need to set up a PCR reaction List all of the ingredients you need to include in the test tube in order for replication to take place A DNA polymerase primers own own template nucleotides Automated gels for DNA analysis Short tandem repeats STR analysis a molecular biology method used to compare specific loci on DNA from two or more samples
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