BIOL 1107 1nd Edition Lecture 19 Outline of Current Lecture I Calvin cycle II C4 Plants III CAM Plants Outline of Current Lecture I DNA Evidence II DNA in Cell III Amplifying DNA with PCR Polymerase Chain Reaction IV History Rosalind Franklin Watson Crick V Components of DNA VI How is DNA Copied Current Lecture I DNA Evidence II Evidence in the courtroom o Blood was previously used for blood typing not most effective method o Now used as source of DNA What cells in blood give you DNA White blood cells Sources of DNA Saliva hair semen DNA comes from sperm urine Uses of DNA fingerprinting Rape murder forensics paternal testing DNA in Cell Any cell in our body that contains nucleus Packaged in chromosomes Double stranded Individual nucleotides Everyone has same genes but different versions of genes X copy different from Y These notes represent a detailed interpretation of the professor s lecture GradeBuddy is best used as a supplement to your own notes not as a substitute III Amplifying DNA with PCR Polymerase Chain Reaction IV Target a region Gel electrophoresis sizing DNA fragments DNA going to separate based on size DNA fragment indicated by the arrow is 450 base pairs in size History Rosalind Franklin Watson Crick V Rosalind Franklin Franklin s X Ray diffraction photograph of DNA Watson Crick saw Franklin s X Ray diffraction helix structure Components of DNA Nucleotides Nitrogenous bases review these o Pyrimidines vs purines o Purine purine too wide o Pyrimidine pyrimidine to narrow o Pyrimidine purine width consistent with X ray data o Strands are anti parallel o Adenine pairs w thymine guanine pairs w cytosine VI How is DNA copied Meselson Stahl experiment Came up w 3 models of DNA replication The experiment provides evidence for the semi conservative replication of the DNA molecule where the two parent strands serve as the template for synthesis of new strands View pictures on next page
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