BIOL 1107 1nd Edition Lecture 12 Outline of Last Lecture I CFTR Protein Cystic Fibrosis II Nucleus III Endoplasmic Reticulum IV Cytoskeleton Outline of Current Lecture I Golgi II Pulse Chase Experiment III Eukaryotes vs Prokaryotes IV Advantages of Organelles V Other Components in the Cell VI Viruses Current Lecture I Golgi packaging and modifying proteins These notes represent a detailed interpretation of the professor s lecture GradeBuddy is best used as a supplement to your own notes not as a substitute II UPS store of the cell side that faces ER cis other side trans exocytosis transport mechanism used to get proteins out of the cell Pulse chase experiment Tagged amino acid methionine serine etc Radioactive can see proteins transferred from ER to plasma membrane in data of tagged proteins Lysosome Intercellular digestion proteins that are malfunction can break down compounds and re use CFTR in non CF patient vs CFTR in CF patient How would a prokaryote make a secreted protein No ER What membrane does prokaryote have Plasma membrane III Eukaryotes vs Prokaryotes Eukaryotes Membrane bound organelles Nucleus 10 100 micro meters size IV Prokaryotes Nucleoid 1 10 micro meters much much smaller Advantages of Organelles Improve efficiency in cells Way of compartmentalizing stuff we don t want harmful substances eating up everything in the cell Extensive number can be used to make lots and lots of proteins Mitochondria uses membrane to make ATP Limits on cell size Yes Q A cell can move a molecule across 1 cm 2 of membrane per minute What final concentration of molecules would be in each of the cells below after one minute molecules cm 3 1 cm 2 cm 3 cm Surface Area 1x1x6 6 2 x 2 x 6 24 3 x 3 x 6 54 Volume 1x1x1 1 2x2x2 8 3 x 3 x 3 27 Concentration 6 1 6 24 8 4 54 27 2 How cells get bigger Villi increase membrane surface area plants use vacuoles egg is it a single cell Yes Egg cell is metabolically inactive V Other Components in the Cell look in the textbook for more detail VI Mitochondria chloroplast energy Peroxisome break down fatty acids H2O2 H2O ECM structure strength elasticity Viruses Extremely small 20 micro meters Life cycle of virus DNA vs RNA virus picture below No smooth ER etc can t make its own proteins steals organelles from host TAMIFLU blocks building off process How do vaccines work view picture on next page RNA not as stable as DNA
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