BIOSC 150 1st Edition Lecture 30Outline of Last Lecture I. Study of EnergyOutline of Current Lecture 1. Harvesting Energy2. Pyruvate ProcessingCurrent LectureAerobic Respiration – getting more out of Glucose Pyruvate processing allows cellular respiration to proceed Pyruvate needs to cross two mitochondrial membranes o Outer membrane – porins, large non-selective channelso Inner membrane – mitochondrial pyruvate carrier Pyruvate processing: decarboxylation, generation of NADH, AcCoAo Oxidation reaction, NAD is reducedPyruvate Acetyl CoA Pyruvate dehydrogenase complex performs a series a complex reactionso E1: 30 subunitso E2: 60 subunitso E3: 12 subunits PDH complex is too big to crystallize; we get an idea of what the structure looks like through cyro-electron microscopy E1, the rate limiting enzyme of PDH complex, is regulated by phosphorylationo Phosphate groups are added to the enzyme through the use of Kinaseo Phosphorylation of E1 renders it inactive therefore turning if ‘off’These notes represent a detailed interpretation of the professor’s lecture. GradeBuddy is best used as a supplement to your own notes, not as a substitute.o Kinase and phosphatase are opposite pairs therefore the use of phosphatase will turn the enzyme back ‘on’ Acetyl CoA feeds into the citric acid cycleCitric Acid Cycle – regenerates product to cycle, generates energy with NADH, FAD, ATP, and decarboxylation of Co2 Loss of CoA = Fuel for the endergonic reactions in the cycle Citric cycle runs twice for each glucose produced Feedback inhibition regulated by NADH and ATPo NADH – competitive inhibitiono ATP – allosteric inhibition Glycolysis and Pyruvate processing fully metabolizes and oxidizes
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