BIOSC 150 1nd Edition Lecture 7 Outline of Last Lecture I. MembranesOutline of Current Lecture 1. Study of diseaseA. Cystic FibrosisCurrent LectureWhat are the symptoms of cystic fibrosis?a) Appearance of being emaciatedb) Sweat is saltyc) Trouble breathingd) Mucous build up in lungsWhat is the cause of cystic fibrosis?a) Hydrophobic chain (trans membrane domains)b) Chlorine ion unable to escape the cell membraneHow to isolate the membrane protein:1. Add detergents2. Binding takes place by the detergent3. Isolation of the protein bound-CFTR (the protein responsible for cystic fibrosis) purification is analyzed by gel electrophoresis-Look at the primary structure (amino sequence) to determine the size of the protein-Purified CFTR is then mixed with phospholipids to make liposomes, the liposomes are then tested for chlorine ion conductance Why is the mucous so thick in patients diagnosed with cystic fibrosis?a) Their CFTR channel is defectiveb) In turn, the chlorine ions are unable to move out the cellThese notes represent a detailed interpretation of the professor’s lecture. GradeBuddy is best used as a supplement to your own notes, not as a substitute.c) This then leads to less movement of water through the celld) Less water leads to more build of sticky mucous How to diagnose the problem of mucous:a) Amylose (carbohydrates) provides digestion of Amylaseb) Lipase (lipids) is added to triglycerides providing digestion of fatty acids and glycerolc) Protease (proteins) provides digestion of amino acids^ All the following enzymes are adding to the patients diet in the form of a pill and are taken during every meal to break down each of the following macromolecules. This helps the patient to gain
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