Lab Exam 1 Review 1 EXPERIMENTATION ANDEXPERIMENTAL DESIGN a Hypothesis tentative explanation i Null hypothesis no effect from independent variable ii Experimental or alternative hypothesis will effect from independent variable b Independent variable variable you manipulate from on experimental group to the next c Dependent variable the variable you measure d Control treatment group the treatment group not subject to the independent variable e Multiple constants anything kept the same from one treatment group to the next f Experimental Treatment group the treatment groups subject to the independent variable g Interpret data to make an inference or draw a conclusion 2 METRIC MEASUREMENT AND CALCULATION OF DENSITY a Always use metric b Always put wiggle digit c Mass grams d Volume cm3 or mL 2 decimals for each i L x W x H cm3 ii Irregular objects Displacement Final volume Initial volume mL e Density g mL or g cm3 i Mass Volume Density f Metric scale chart pg 52 g 3 MOLAR SOLUTIONS a Preparing A stock solution required volume in L x required molarity in M x mol Wt of compound b Ex 3 liters x 0 5 M x 95 21g mole of MgCl 2 142 82g of MgCl2 is needed c 4 BIOCHEMICAL TESTS USING REAGENTS a Sugar Reducing sugar Simple Carbohydrates Benedict s reagent heat positive color change is green to dark orange b Starch polysaccharide Complex Carbohydrate Lugol s Iodine mix positive color change is dark blue black c Protein Biuret reagent mix positive color change is violet d Lipids paper strip opaque neg translucent stain clear pos e When stating results say what is present and not present 5 MICROSCOPE CELL RECOGNITION AND DOCUMENTATION pg 106 107 a b Label two or more features structures and properly document your specimen using proper drawings i LOOK AT PAGES 106 108 ii Bacteria 1 Shapea Cocci spherical b Bacilli rods c Spirilla spirals 2 Arrangementa Diplo pairs b Strepto chains c Staphylo clusters c Calculate total magnification i Total Obj x Ocular d Identify specimen e State what type of cell Eukaryote or Prokaryote f Identify domain i Eukaryotes Eukarya larger w nuclei and membranous organelles ii Prokaryotes Bacteria or Archaea lack nucleus and lack membranous organelles 6 OSMOLARITY AND GRAPHING a b change in mass final intial intial x 100
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