Chapter 8: Lymphocyte Development and Antigen Receptor Gene Rearrangement (Part 2) Junctional Diversity - P Nucleotides vs. N Nucleotides B Cell Development – aka. How do we make a mature, functional B cell? Abbas, Lichtman and Pillai8_Figure 8-11; p.184 Abbas, Lichtman and Pillai8_Figure 8-12; p.185Pro-B: Pre-B: Since we have two chromosomes, we get two chances to rearrange the DNA but only one heavy chain gene will be expressed (allelic exclusion); if the first rearrangement does not produce a functional receptor, we can use the other chromosome. However, if we make a functional receptor on the first try, rearrangement on the other chromosome is inhibited. Abbas, Lichtman and Pillai8_Figure 8-13; p.186Formation of the Pre-BCR Formation of the light chain Abbas, Lichtman and Pillai8_Figure 8-13; p.186 Abbas, Lichtman and Pillai8_Figure 8-14A; p.187Immature B cells Mature B Cell Types Abbas, Lichtman and Pillai8_Figure 8-15; p.189 Abbas, Lichtman and Pillai8_Figure 8-13; p.186Alternative mRNA splicing T Cell Development– aka. How do we make a mature, functional T cell? Abbas, Lichtman and Pillai8_Figure 8-16; p.189 Abbas, Lichtman and Pillai8_Figure 8-17; p.191This process is similar to the maturation and development of B cells, but there are some differences. Please recall that the thymus is the location of T cell maturation and these developing T cells are more generally called thymocytes. Please read the last two paragraphs on p.191 (beginning with “The movement of cells…”) and identify the chemokines and chemokine receptors involved in regulating the movement of T cells through the thymus. An overview of T cell development Double-negative thymocytes (Pro-T cells) Abbas, Lichtman and Pillai8_Figure 8-18; p.192 Abbas, Lichtman and Pillai8_Figure 8-19; p.193Pre-T cell The Pre-T Cell Receptor The function of the pre-TCR is to make sure the chain is functional. If it is functional, the pre-TCR will: 1) 2) 3) 4) Please read the section on “Pre-T Cell Receptor” on p.193 and 194 to find the answer to the above. Abbas, Lichtman and Pillai8_Figure 8-14B; p.187 Abbas, Lichtman and Pillai8_Figure 8-19; p.193Double-positive thymocytes Single-positive thymocytes (immature T cell) Positive and negative selection Mature T Cell Abbas, Lichtman and Pillai8_Figure 8-19;
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