BIBC 102: Metabolic Biochemistry (Prof. Scheffler) TA: Edward Chen [email protected] Section: Mondays 4-5pm Center 218 OH: Wednesdays 10-11am @ CLICS Handout #5 (3/6/06) A. Gluconeogenesis • The formation of glucose from non-hexose precursors. • This is the opposite of glycolysis. • 3 different bypasses: B. Pentose Phosphate Pathway • To generate NADPH for anaerobic reactions and ribose to synthesize DNA and RNA C. Photophosphorylation • Chloroplast – • Things to know in chloroplast: Grana – Stroma – Outer membrane – Inner membrane – Thylakoids – • Plants use photon light to create energy First degree pigments Second degree pigments • Light Harvesting Complex (LHC) Captures photons and bring them to the reaction centers Each functional unit is a LHC trimer with 36 chlorophyll and 6 lutein molecules Each α-helical segment has 12 chlorophyll (7 chlorophyll a and 5 chlorophyll b) and 2 molecules of accessory pigment luteinBIBC 102: Metabolic Biochemistry (Prof. Scheffler) TA: Edward Chen [email protected] Section: Mondays 4-5pm Center 218 OH: Wednesdays 10-11am @ CLICS • Fate of Photon Internal conversion: Fluorescence: Exciton transfer: Photooxidation: • Photochemical Reaction Centers – Oxygen evolving complex Photosystem I and II (write this on the back side o.O) Overall Reactions Non-cyclic: 2H2O + 8 photons + 2NADP + ~3 ADP + ~3 Pi → O2 + ~3ATP + 2NADPH Ö Cyclic: ADP + Pi → ATP + H2O Ö • Spatial separation of PSI and PSII prevents exction larceny (pg. 736) • Comparison between mitochondria, chloroplast, and
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