Name (last, first) BIBC 102 (METABOLIC BIOCHEMISTRy) Dr. Lukas Buehler Summer Session 01, 2008 FINAL EXAM August 2, 2008 TOTAL 140 POINTS All Students please read the following waiver: By signing this waiver, I give permission for this exam to be left for me to pick up in Bonner Hall outside office 3230. IfI do not sign this waiver, I acknowledge that my exam will be available for pickup during office hours or class. ----------_/_------Signature Question # Points Question # Points I (10 points) 8 (10 points) 2 (10 points) 9 (l0 points) 3 (10 points) 10 (10 points) 4 (10 points) 11 (10 points) 5 (10 points) 12 (10 points) 6 (10 points) 13 (10 points) 7 (10 points) 14 (10 points) ----Total (140 points)Name (last, first) _ 2 1. What is its common name of the fatty acid shown below? Name the two types of reactions required to synthesize this molecule from palmitoyl-CoA. Put them in the correct order and name the farty acid intermediate. Indicate where in the cell these reactions occur. lap CI8:1 cis~9 ~ ~ dl/Vl~ at; ~ j t j ~-r--r<'".......,"' ~ ~ Cri ·'·0 2. Name the C3 substrate for cytoplasmic farty acid synthetase. What is the name ofthe subunit of the enzyme complex that binds this C3 compound during fatty acid synthesis? How many C3-CoA units go into the synthesis of one molecule of palmitoyl-CoA? lap !luA-tCfr l( V\~+--gI-l-,-ui-t ~J .~ 0~1!-I'J M",{ r--jJ La A-)~ \') vco-t ~Cv~"JName (last, first) _ 3 3. What is the role of leghemoglobin in root nodules of Rhizobium containing plant roots? What would the consequence be of an inactive leghemoglobin protein? lap ~l~ <.. l--\t 'fwt)f ,1a ~ov..z de c_ C/'--rL~ ~~J fs out c Ir<~-7 CJLI tA~~ ~ ~} (N XI/1 ~ ~:gn~)) 4. Name the metabolic precursor in the synthesis of the aromatic amino acid tyrosine: , lap a) in human b) in E.coli (use the last branching point intermediate) r rad"v{ C--,~l-L t.J \./~ Q.j I ~ 5. Name the reactions (or enzymes) in beta-oxidation that produce high-energy compounds that can be used by the mitochondrial electron transport chain. Also name these high-energy compounds. lOp lName (last, first) 4 6. What is the net reaction catalyzed by the enzyme glutamate dehydrogenase (no chemical structures needed)? lOp ", 7. Ornithine belongs to which class of metabolites? It is involved in which metabolic pathway (name only, no outline of pathway)? "Which ketoacid intermediate of this pathway provides a link back to the Krebs cycle? lOp (.:~~o-it 0 illV~ WCv ~ ~--t-r ~ASl~ 8. Discuss two ways of synthesizing phosphatidylcholine (one is preferred in mammals, one in bacteria and yeast). lOp --_.-Name (last, first) _ 5 9. How does cysteine synthesis in human affect the activated methyl cycle? What amino acid would be needed in your diet to minimize the effect ofcysteine synthesis? lOp t 10. Explain how the conversion from serine to glycine is linked to DNA synthesis. lOp ------l (j fVVv~V\-c ~ I • c..., ./~Name (last, first), _ 6 11. Discuss the regulation of acetyl-CoA carboxylase when glucose availability and blood glucose levels are high. lOp f) \~k~ JA-j [ 12. What is the ro Ie of malic enzyme and which alternate pathway can a cell use if this enzyme is not active, but the cell needs to synthesize lipids? lOp ......oe.. ---~/~Ib t5Ct 7A/IL L-·r t{Name (last, first) _ 7 13. Which class of enzymatic reactions catalyzes the formation of cholesterol from squalene, and cholesterol to pregnenolone? Name the enzyme type and outline the general net reaction it catalyzes. lOp 14. What is the role ofthioredoxin in DNA synthesis?
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