1 Name (last, first) ~ yc------BIBC 102 (METABOLIC BIOCHEMISTRY) Dr. Lukas Buehler Summer Session 01 2008 MIDTERM EXAM 1 July 102008 TOTAL 100 POINTS All Students please read the following waiver: By signing this waiver, I give pennission for this exam to be left for me to pick up in Bonner Hall outside office 3220. If I do not sign this waiver, I acknowledge that my exam will be available for pickup during office hours or class. -----------_/_------Signature Date I_Q=-.-ue_s_ti_o_D_# I_P----'o'---in_t_s _ 1 (10 points) 2 (10 points) 3 (l0 points) 4 (10 points) 5 (l0 points) 6 (10 points) 7 (10 points) 8 (10 points) 9 (10 points) 10 (10 points) Total (100 points)2 Name (last, first) _ 1. Name the possible metabolic fates (pathways or products) of glucose-6-phosphate in a liver cell and indicate if they are likely to occur under conditions of high or low-energy charge. 10 points C,&L~r rlrl~ l{ ~~ ~ &--r:--.,-(9 p ~') ~ q \) ~ [OW f~(v?tx 2. List the four coenzymes or high-energy compounds that have ADP as part oftheir structure. Indicated which one plays a role in the reaction catalyzed by lactate dehydrogenase. 10 points C, It ftlf 11 t\'P-t{ (+it f AlP«~Name (last, first) _3 3. Which glycolitic step couples a redox reaction with the formation of new high-energy phosphate bond that does not involve ATP/ADP? Give the full reaction of this step, the name of the enzyme catalyzing .. the reaction and indicate the molecule that is the donor of the reducing equivalents exchanged in this redox reaction. 10 points 4. What is the thermodynamic criterion for reversibility of reactions? Why are reactions that are predicted to be irreversible under standard conditions perfectly reversible under cellular conditions? 10 points S~~ 0 ~W-Cd\ACRN< ~'ow ~ ~~+ ~ ?>lv.-kl ~li\~ ~j4 Name (last, first) _ 5. What is the net result in energy for the pentose phosphate pathway for the complete oxidation of one molecule of glucose into six carbon dioxides? What is the role of the non-oxidative branch under this condition? 10 points 6. Forming high-energy compounds is often the first step in pathways. What high-energy formes) of glucose is/are made during glycogen synthesis? 10 points5 Name (last, first) _ 7. Which glycolytic enzyme is inhibited by acidic conditions (protons)? What other allosteric inhibitor(s) do you know for this enzyme? 10 points ( 8. Describe the Con cycle. Which cell types are involved? What are the starting substrates and products in each cell type (do not explain pathway details or control mechanisms)? What are the energy benefits/costs for the cell types involved? 10 points t~A-~> _ '2 tJrt'D~ /« ~(wt6 Name (last, first) _ 9. How does the first step of glycolysis differ in E.coli and human muscle cells? 10 points 10. Explain the meaning of feedback and feed-forward regulation in metabolic pathways (you can use generalized pathway features), and show how feed-forward regulation operates in glycolysis. 10
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