Spring'2011'BIBC'102'final' ' Name__________ Bongo Metabolondo'__________'Hampton'Class'@'6:30'T,'Th' ' ID'#_______________________________________________'' ' ' '1'! Good evening! This is the final, for the Metabolic Biochemistry class. Even though you may not feel it, you have learned a lot and will now view the living cell in a whole new way. Not bad for 19 dinner hours. This test will be handed in, and will be available for pickup sometime next week. The answer key will be on the website TONIGHT, and you will do yourself a favor to register any complaints, comments, questions, or corrections to me by email (don’t use the text number for this). We just may fold those suggestions into the grading… I have enjoyed teaching you metabolism. As a small token of this, and to commemorate our own metabolisms, I am going to have a bunch of pizza outside the test room, which you can eat. I will eat some too. Good luck and have a great Summer! Summation Page 2 _________(out of 10) Page 3 _________(out of 15) Page 4 _________(out of 13) Page 5 _________(out of 11) Page 6 _________(out of 15) Page 7 _________(out of 10) Page 8 _________(out of 15) Page 9 _________(out of 8) Page 10 _________(out of 10) Page 11 _________(out of 12) Page 12 _________(out of 10) Total ___________(out of 129) adjustment to 150 (score time x 150/129) _________Spring'2011'BIBC'102'final' ' Name__________ Bongo Metabolondo'__________'Hampton'Class'@'6:30'T,'Th' ' ID'#_______________________________________________'' ' ' '2'!! Enzymes and Energetics (10 pts) –– B ––– 1) Enzymes alter A) The equilbirum constant of a reaction B) The rate of a reaction C) The free energy of a reaction D) All of the above –– C ––– 2) The kcat for an enzyme A) changes with experimental conditions B) is the concentration of substrate at half maximal rate C) is the maximum reactions per second per molecule D) is the ratio of the rate and the substrate concentration –– B ––– 3) The Km for an enzyme A) changes with experimental conditions B) is the concentration of substrate at half maximal rate C) is the maximum reactions per second per molecule D) is the ratio of the rate and the substrate concentration –––– D –– 4) If the ΔG‡ goes down by the quantity d A) The rate of a reaction will slow by a factor of e to the (d/RT) B) The reaction will go further to the right C) The reaction will go further to the left D) The rate of reaction will increase by a factor of e to the (d/RT) –– T ––– 5) T/F: allosteric enzymes do not obey Michaelis Menton kinetics –– T ––– 6) T/F: adenylation is a modification used to regulate enzyme activity –– F ––– 7) T/F: regulatory phosphorylation always causes enzymes to have higher activity –– F ––– 8) T/F: Two reactions with positive ΔG when run together have a negative ΔG –– F ––– 9) T/F: NAD+ reduction and NADP+ reduction have very different E'o 10) Does the Lineweaver-Burke plot show a group of enzymes with the same Km or the same Vmax? Or neither? Say which, and why you say it (one sentence) Same Km: the different curves all have the same X intercept, meaning they all have the same Km (since intercept equal -1/Km)Spring'2011'BIBC'102'final' ' Name__________ Bongo Metabolondo'__________'Hampton'Class'@'6:30'T,'Th' ' ID'#_______________________________________________'' ' ' '3' Breaking and Making Glucose (15 pts) ––– A ––– 11) Glycolysis A) Is exergonic, that is, it has a negative ΔG B) Is endergonic, because it produces high energy ATP C) Is endergonic because it requires ATP hydrolysis D) Has a zero ΔG as a process because it is in equilibrium ––– B ––– 12) Gluconeogenesis A) Occurs by every glycolytic step running backwards B) Occurs by many glycolytic steps running backwards C) Converts pyruvate into PEP in a single step D) Has a positive ΔG for the whole process ––– B ––– 13) Glycolysis A) Produces more ATP than full oxidation of glucose B) Produces ATP faster than oxidation of pyruvate C) Only functions to feed the mitochondrion oxidative fuel D) Occurs in the mitochondrion ––– D ––– 14) The pentose phosphate pathway A) produces a lactone B) generates NADPH C) releases CO2 D) all of the above ––– B ––– 15) Pyruvate carboxylase A) Is an enzyme of glycolysis B) Is an enzyme of glUconeogenesis C) Is an enzyme of both glycoylysis and gluconeogenesis D) Is involved in neither glycolysis and gluconeogenesis ––– F ––– 16) T/F: No animals depend only on glycolysis for glucose energy ––– F ––– 17) T/F: Galactose is not metabolized by glycolysis ––– T ––– 18) T/F: Glycerol can be used to make glucose 19-24) (3 pts total) The following five molecules are substrates of the first five reactions of gluconeogensis. Put them in order for that process. Starting with pyruvate ___4__ 2PG ___3__ PEP ___1__ Pyr ___5__ 3PG ___2__ OAA 25) (4 pts) Write the reaction for GAPDH (glyceraldeyhe-3-phosphate dehydrogenase) including structures of the glucose-derived moleculesSpring'2011'BIBC'102'final' ' Name__________ Bongo Metabolondo'__________'Hampton'Class'@'6:30'T,'Th' ' ID'#_______________________________________________'' ' ' '4' Krebs and related Turns of Metabolic Fate (13 pts) ––– B ––– 26) The pyruvate dehydrogenase complex A) Converts pyruvate into lactic acid B) Generates CO2 and carrier-bound acetyl groups C) Oxidizes pyruvate into phosphoenoyl pyruvate D) Uses lipoic acid to carboxylate pyruvate ––– D ––– 27) The Krebs cycle A) Is an anabolic process B) Is a catabolic process C) Is neither an anabolic nor a catabolic process D) is both a catabolic and anabolic process ––– A ––– 28) alpha ketoglutarte dehydrogenase A) uses lipoic acid as a cofactor B) does not change the carbon number between substrate and product
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