Lecture 16: FertilizationPowerPoint PresentationCapacitationChang Experiment - 1951Slide 5Effect of CapacitationModel of CapacitationSlide 8CapacitationThe Acrosome ReactionSlide 11The Zona PellucidaSperm Interaction with the ZonaSlide 14Slide 15Slide 16Slide 17Maturation Promoting Factor (MPF)Slide 19Slide 20Initiation of Calcium Pulse by Soluble Sperm ComponentSperm Receptor - Calcium Pulse InitiationSlide 23Slide 24Slide 25Blocks to PolyspermySlide 27Slide 28Slide 29Slide 30Lecture 16: FertilizationAnimal Science 434John J. ParrishCapacitation1951Austin - ratChang - rabbit Fertilization does not occur until several hours after sperm are placed in the uterus or oviductChang Experiment - 1951020406080100-2 -1 0 2 4 6 8Time of Insemination (hr relative to ovulation)OvulationOvulationPercent FertilizationAfterAfterBeforeBeforeCapacitationThe changes within sperm that confer upon the sperm the ability to acrosome react in response to the appropriate stimulus.Zona PellucidaCumulus CellsFollicular FluidZona PellucidaCumulus CellsFollicular FluidEffect of CapacitationEpididymal EjaculatedSeminalPlasma+CapacitatedFemaleTract+Model of CapacitationD FPlasma MembraneCytoplasmNucleusAcrosomeAcrosomeD FPlasma MembraneCytoplasmNucleusAcrosomeAcrosomeGAGCholesterolChol-AcceptorCa2+Ca2+pHcAMPActivity ofEnzymesActivity ofEnzymesD FD FD FD FCapacitation No change in sperm morphologyMotility change–hyperactivationcAMPPower outputcAMPPower outputThe Acrosome ReactionPlasma MembraneAcrosomeFused PlasmaMembrane and Outer AcrosomalMembraneFused PlasmaMembrane and Outer AcrosomalMembraneNucleusAcrosinHyaluronidaseAcrosinHyaluronidaseModificationof the EquitorialRegionModificationof the EquitorialRegionThe Zona PellucidaThe Zona PellucidaZona PellucidaZP3AttachmentBindingAcrosome ReactionInductionAcrosome ReactionSperm Interaction with the ZonaZona PellucidaZP2ZP2ZP2ZP2Secondary BindingZona PenetrationMotilityMotilityZona PellucidaSperm tract through zona pellucidaSperm tract through zona pellucida6-12 hr6-12 hr18-24 hr18-24 hrMaturation Promoting Factor(MPF)Metaphase Promoting FactorMetaphase Promoting FactorMPFMPFMPFMPFPrimary OocyteGV-IntactGVBDMetaphase IPB-1Secondary OocyteMetaphase IIOvulationLH SurgeZona PellucidaPerivitellineSpaceOocyteCa2+Ca2+Ca2+Ca2+9080706050403020100050100150200250300350400450500Time (m in)Calcium (nM)9080706050403020100050100150200250300350400450500Time (min)Calcium (nM)9080706050403020100050100150200250300350400450500Time (min)Calcium (nM)9080706050403020100050100150200250300350400450500Time (min)Calcium (nM)Calcium Transients During Bovine FertilizationCalcium Transients During Bovine FertilizationInitiation of Calcium Pulse by Soluble Sperm ComponentEndoplasmic ReticulumCa2+PLCzetaPIP2DAGIP3Ca2+OocyteSperm Receptor - Calcium Pulse InitiationCa++Ca++ERERIP3IP3PLCPLCZona PellucidaPerivitellineSpaceOocyteMIIAIIMPF XCa2+Ca2+PronucleiTIIPBIIZona PellucidaPerivitellineSpaceOocyteMIIAIIMPF XCa2+Ca2+PronucleiDecondensationPronucleiZygoteTIIPBIIZona PellucidaPerivitellineSpaceOocyteCa2+CorticalGranulesZona Block to PolyspermyBlocks to Polyspermy1234Species1.Mammals3.InvertebratesAmphibiansFishRabbits4.AmphibiansAviansSperm acrosomalregion binds to spermreceptor on zonaSperm penetrateszonaInitiates -Zona &VitellineReactionInitiates completion ofMeiosis II and releaseof 2nd polar bodyZona and VitellineReactions blockpolyspermyPerivitellineSpaceVitellineMembraneCorticalGranuleZP3BindingInitiation ofAcrosomeReactionContinuation ofReactionAcrosome- ReactedSpermPenetrationExcluded SpermZonaReactionCorticalGranuleReleaseFusion1st Polar BodyZonaPellucidaRedrawn fromWasserman, P.M. 1988 Scientific American.
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