Animal Science 434Sperm Transport in the Male TractSlide 3Slide 4Site of EjaculationSperm Loss From Female TractExperiment on Retrograde Flow in the CowPowerPoint PresentationSlide 9Evolutionary Strategies for Dealing With Retrograde Flow of SemenSlide 11Neutrophile Infiltration of UterusSlide 13Slide 14Effect of Oviduct Transection on Fertilization in HeifersSperm Transport in the PigSlide 17Slide 18Slide 19Slide 20CervixSlide 22Slide 23Slide 24Slide 25Slide 26Factors Regulating Oocyte Transport in the OviductSlide 28Slide 29Slide 30Slide 31Animal Science 434Lecture 15: Gamete TransportSperm Transport in the Male TractFluid FlowCiliaFluid FlowMuscle ContractionsSperm Transport in the Male Tract•No protein synthetic ability•addition or loss of plasma membrane proteins and lipids•redistribution of proteins and lipids within sperm•changes in lipid diffusion coefficients Acquisition ofFertilizing Abilityand MotilitySperm Transport in the Male TractAddition of Decapacitation FactorAddition of DF From Accessory Gland SecretionsDecapacitation Factor•protein, glycolipid and/or lipid•stabilizes the plasma membraneSite of EjaculationSite of ejaculation Semen Characteristics SpeciesVagina slight coagulation of ejaculate human, rabbitvagina semen with high sperm cattle, sheepconcentrationuterus voluminous, distention of cervix horsecervix, uterus voluminous, retention of penis dog, pigduring copulationuterus vagina plug rodentsSperm Loss From Female Tract•retrograde flow•phagocytosis by neutrophilsExperiment on Retrograde Flow in the CowUterine HornUterine BodyCervixInsemination at 3 different spots05101520Cumulative % Sperm Recovered From Vagina0 1 2 3 4 5 6 7 8Hour After InseminationUterine BodyUterine HornCumulative % Sperm RecoveredFrom the Vagina0102030405060cervix0 1 2 3 4 5 6 7 8Hour After InseminationCumulative % Sperm RecoveredFrom the VaginaHours After InseminationCervixUterine HornEvolutionary Strategies for Dealing With Retrograde Flow of Semen•coagulation of semen or a plug•concentrated semen•semen deposited in uterusSperm Loss From Female Tract•retrograde flow•phagocytosis by neutrophilsNeutrophile Infiltration of UterusPhases of Sperm Transport•rapid »15-30 min•sustained»6 - 12 hr•oviductal»at ovulation•rapid »15-30 min•sustained»6 - 12 hr•oviductal»at ovulationEvidence for Sustained TransportLigatures atUtero-tubal JunctionLigatures atUtero-tubal JunctionEffect of Oviduct Transection on Fertilization in HeifersInterval from mating* to Oocytes Fertilizedtransection of UTJ6 0%8 50%12 83%* Mating was done at the start of estrus.454035302520151050020406080100Effect of Isthmus Transection on Fertilization in the PigTime From Mating to Transection (hr)Percentage of Oocytes FertilizedControl (no transection)Transected IsthmusOvulationIsthmusUterusLigationto Transectthe IsthmusAmpullaOvarySperm Transport}2 cmSperm Transport in the PigPercentage of Oocytes Fertilized}OvulationOocyteCumulus Cells Follicular FluidCue for ovulation may effect isthmus to trigger oviductal transport6-12 hr18-24 hr1-2 hrLocation of Fertilizing SpermBarriers To Sperm TransportCervixBarriers To Sperm TransportUterotubal JunctionOvulationOocyteCumulus Cells Follicular FluidFluid FlowFluid FlowFactors Regulating Oocyte Transport in the OviductRemains closed untilday 3 - 5 post ovulationToward UterusTowardAmpullaTotal Fluid ProductionFactors Regulating Oocyte Transport in the OviductCiliaFluid FlowFluid FlowMuscleContractionsNo ContractionsRemains closed untilday 3 - 5 post ovulationSite ofFertilizationSite
View Full Document
Unlocking...