BSC 160 1nd Edition Lecture 18 Outline of Last Lecture 1 Turbidostat 2 Environment Growth 3 Philes Outline of Current Lecture 1 Genome 2 Mutation 3 Gene Transfer Systems Recombination Current Lecture Chapter 12 Genomics Genetics Genome Important information of functioning Wild type wt Mutant Benchmark Genotype Genetic makeup Specific sequence of nucleotides Phenotype Physical characteristics that you can see Mutations Tight cannot be grown in absence of whatever they need Display mutant phenotype clearly Leaky Displays phenotype slightly different from wild type Stable or Unstable Frequency at which mutation will revert back to wild type on its own Stable good physiological condition Doesn t revert back or very infrequently UnstableReversion Returning to wild type Conditional Mutations Express mutant phenotype under certain conditions When you see wt under permissive conditions Express mutant wt non permissive conditions His or His PrototrophAuxotroph How do we approach Selection Selecting Media Screens looking colony by colony hope to grow what you want to grow Time consuming Why Make Mutants Gene Transfer Systems Recombination Genetic recombination occurs when an organism acquires expresses genes that originated in another organism 3 means for genetic recombination in bacteria 1 Conjugation 2 Transformation 3 Transduction Conjugation Transfer of plasmid or chromosomal fragment from donor cell to a recipient cell via direct connection Transformation Chromosome fragments from a lysed cell are accepted by a recipient cell the genetic code of the DNA fragment is acquired by the recipient Transduction Bacteriophage serves as a carrier of DNA from a donor cell to a recipient cell
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