BioSc 231 General Genetics Exam 5 Name Multiple Choice 2 points each When an organism gains or loses one of more chromosomes but not a complete haploid set the conditions is known as A polyploidy B euploidy C aneuploidy D triploidy E trisomy It was once thought that the karyotype was related to criminal disposition because it led to aggressive behavior due to excessive maleness A XO B XY C XXY D XYY E YY Differing sizes of restriction fragments produced from the alleles of a gene constitute A a southern blot B an allozyme C identification of a gene D a restriction fragment length polymorphism Which of the following is NOT necessary in order for a population to maintain Hardy Weinberg equilibrium A random mating B high rate of migration C large population size D allele frequencies are the same in males and females In a population in Hardy Weinberg equilibrium the frequency of the allele for Hartnup disease is 0 008 What percentage of the population is homozygous for this allele A 0 064 B 0 0064 C 0 008 D 99 E 0 01 In a population with two alleles B and b the frequency of b is 0 56 What would be the frequency of the heterozygotes assuming Hardy Weinberg equilibrium A 0 55 B 0 36 C 0 71 D 0 31 E 0 49 Which of the following processes does not contribute to changing allele frequencies in populations A mutation B inbreeding C migration D natural selection E genetic drift Enzyme that cleaves DNA at sequence specific sites is called A DNA polymerase B ligase C restriction endonuclease D sticky ends E cDNA DNA termini without overhangs produced by endonuclease digestion are called A cohesive termini B sticky ends C blunt ends D oligonucleotides E none of the above Which enzyme functions to synthesize RNA from a DNA template A DNA polymerase B RNA polymerase C reverse transcriptase D ligase E endonuclease Which vector element below can accept the largest DNA fragment in cloning experiments A plasmid B bacteriophage lambda C YAC D cosmid To prepare clones representing expressed genes from a eukaryotic cell you would mix total cellular mRNA with A reverse transcriptase B RNA polymerase C DNA polymerase D integrase E RNAse Polyploid plants found in nature usually have even numbers of chromosomes because organisms having odd numbers A exhibit altered mitosis B are unable to asexually reproduce C exhibit altered growth D have low fertility E are not viable Pollen from one species germinates on the stigma of another related species and sexually fertilizes the ovule Most of the resulting plants are sterile but some of the resulting offspring undergo chromosome duplication resulting fertile plants The fertile offspring are known as A hexaploid B autopolyploid C monoploid D diploid E allopolyploid In a mammal how many inactivated X chromosomes Barr bodies would be present in cells of individuals who were XXX A 0 B 1 C 2 D 3 E 4 When an allotetraploid is backcrossed to one of its progenitor species a sterile progeny is produced The genomic composition of this sterile individual can be best represented by A n1 n2 B 2n1 2n2 C 2n1 D 2n2 E 2n1 n2 Euploidy is A a chromosome number that is not an exact multiple of the haploid number B an example of aneuploidy C a chromosome number that is an exact multiple of the haploid number D the addition of an extra copy of a particular chromosome E the absence of a particular copy of a chromosome Calico cats have a patchwork pattern of yellow and black spots This pattern is an example of A somatic mutation B dosage compensation C mitotic segregation D all heterozygotes display this phenomenon regardless of sex E mosaicism The basic diploid chromosome number in Poinsettia is 16 A new species of Poinsettia is found that has four complete sets of the chromosomes found in the 16 chromosome species This species is said to be A haploid B diploid C triploid D tetraploid E pentaploid Consider a species with a diploid 2n number of 10 chromosomes How many chromosomes would be found in a trisomic body cell A 9 B 10 C 11 D 20 E 21 Consider a species with a diploid 2n number of 20 chromosomes How many chromosomes would be found in a monoploid body cell A 10 B 20 C 21 D 19 E 40 How many different trisomics could be formed in a plant with a diploid number of 20 A 10 B 20 C 21 D 19 E 40 Mosaicism is due to A X inactivation B chromosome duplication C chromosome deletion D somatic mutations E gametic mutations A collection of plasmids containing foreign DNA fragments representing the entire genome of an organism is called a n A transgenic B cDNA C clone D vector E library Problems 3 points each You want to design an oligonucleotide probe to identify a clone containing a new enzyme that you purified You determine that the amino terminal sequence of your enzyme is MCFYMDW What should be the sequence of the oligonucleotide probe Indicate redundancy by putting all possible nucleotides for a particular position in parenthesis In a restriction mapping experiment using the plasmid PST you cleave the circular plasmid with the individual restriction enzymes XbaI Asp718 BamHI and EcoRI individually and in combination with one another and obtain restriction products of teh sizes shown below Enzyme s used Sizes of DNA fragments produced bp BamHI EcoRI XbaI 3 000 1 550 Asp718 BamHI XbaI 3 000 2 500 600 Asp718 BamHI 5 500 600 Asp718 XbaI 3 600 2 500 BamHI XbaI 3 100 3 000 Asp718 6 100 BamHI 6 100 XbaI 6 100 Using the above data make a restriction map of the PST plasmid indicating distances between adjacent restriction sites Bertie is married to Matilda who was previously married to Wynton now deceased Wynton and Matilda conceived one child together and adopted one child Bertie and Matilda have also conceived one child All members of Matilda s current family have had DNA fingerprinting done at a single VNTR locus Unfortunately the cheet that identified each child has been misplace Identify which fingerpring in each lane in lanes 5 6 and 7 correspond to each child Berti3 and Matilda s conceived child Child Wynton and Matilda s conceived child Child Wynton and Matilda s adopted child Child To the right is a hypothetical autoradiogram of a dideoxy sequencing gel What is the sequence of the gene fragment Be sure to label the 5 and 3 ends