Stage 2 - 1st TrialStage 2 - 2nd TrialPhase 2 - 2nd Trial Successes & ProblemsPhase 2 – Experimental ProcedurePhase 2 – Channel LayoutProcessing ProblemsProcessing Problems ContinuedStage 2 - 1st TrialProblems•Thickness of PDMS layers•Interconnects •Delamination•Air bubblesStage 2 - 2nd TrialFabrication•Successfully made and aligned four layers•Layers had very few defects•All interconnects joined two different layers•Entire wafer looked very good- no rough edges, no air bubbles between layers, no cratersPhase 2 - 2nd TrialSuccesses & Problems•Liquid to flow in all channels–all the way through 2 out of 5 channels•Tracked fluid flow using bright food coloring•Tested the effects of vertical interconnects•No capillary action–had to use pressure from syringe•problems•Pressure caused delamination•Effects of vertical interconnectsPhase 2 – Experimental ProcedurePhase 2 – Channel LayoutProcessing Problems•Substantial amount of cracks in SU-8 layer•Layer alignment problems–Razor blade/ tweezers method–Layer thickness–Wrinkles–Air pocketsCracks in reservoir region of SU-8 moldProcessing Problems Continued•Feature alignment–Extremely difficult–Inaccurate•Interconnect layer–No connection provided–Problem with layer
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