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Experimental ObservationsNovember 4, 2003Multilayer microfluidic channel-4 layer PDMS-without SDSChannel Set 1 (Green)Attempt 1: First inserted the needle into IN1.Results: No fluid was injected.Attempt 2: Injected fluid via OUT1Results: DelaminationChannel Set 2 (Yellow)Attempt 1: Injected the fluid through IN 2.Results: The fluid went along the channel for a short distance but broke through the channel creating a fluid bubble under all the layers.Attempt 2: Injected via OUT 2b.Results: The water travel through the channel and stopped at the interconnect.Attempt 3: Injected via OUT 2aResults: The water traveled all the way across the channel and exited out of OUT 2b. Channel Set 2 was designed to test multiple levels and unfortunately the water didnot travel through the interconnect so no water made it to IN 2. (why…was it blocked or just didn’t work)Channel Set 3 (Red-Orange)Attempt 1: Injected via IN 3.Results: The water traveled through half the channel and then delamination occurred. This caused some fluid to appear in the well IN 2 which told us the layers fully delaminated and the liquid no longer was contained within the channel.Attempt 2: Injected via OUT 3.Results: The fluid traveled all the way across the channel but only when we pressed our fingers on either side of the channel to close the separating layers.Channel Set 4 (Red-Orange)Attempt 1: Injected via In 4.Results: Fluid crawled along the channel. Failure occurred at the interconnect, the pressure built up and the layers delaminated.Channel Set 5 (Green)Attempt 1: Injected via OUT 5.Results: It was a success. But there was a lot of backflow causing a bubble for form at the surface where the liquid was being injected.General Observations and Conclusions-One of the largest difficulties was the seal between the needle and the wells of the device. The tip of the needle had a strong slant. This caused us to place the needle at the difficult angle and push the needle partially through the channel. Since the needle was wider than the channel the layers began to separate. A finger was placed on the needle to place a seal, and sometimes we pushed from the top to prevent delamination. -We also saw no capillary action.-The seal between the PDMS and Si wafer was worse than the seal between the PDMS-PDMS


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UMD ENMA 490 - Experimental Observations

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