Name: Student ID: General Biology Laboratory Exercise Lab Exercise 2MicroscopyProper handling of microscopesAlways use 2 hands when picking up the microscopes with one hand under the base and one hand holding the arm. Do not drag the microscope across the counters Always use lens paper and lens cleaner to clean the objectives or oculars.All microscopes are numbered. Always place the microscope in its correct numbered slot in the cabinet. The cord must be wound correctly and the microscope must be left with the low power objective down for storage. Always locate your specimen using the coarse adjustment first, and then use the fine adjustment to get best resolution. Once your specimen is in focus, you can switch to another objective without having to start all over again with the coarse adjustment. ONLY USE THE COURSE FOCUS UNDER LOW POWER. ONCE IN COURSE FOCUS YOU ONLY USE FINE FOCUS!! The course focus moves the stage up and down. If you use the course focus under the higher power objectives then you run the risk of cracking and breakings slides. Course focusMoves stage upSlideBroken Slidemolecules. Describe the following types of Microscopy:DissectingMagnification RangeLightMagnification RangeScanning electronTransmission electronWhat type of microscopy is used below?Microscopes allow one to view objects that are not visible with the naked eye. Since their invention in the 1600s, they have opened up new levels of detail and complexity that were not known to exist. And depending on the type of microscope, they allow us to see inside of organisms, cells andHow a microscope worksA discussion of the electromagnetic spectrum. Now that you have had a discussion of the electromagnetic spectrum answer the following questions1. What type of radiation is damaging to DNA and capable of causing mutations?2. What type of radiation is used for cancer therapy and why?3. What is the resonance frequency of microwave radiation? Is a microwave capable of causing mutations?4. How is wavelength related to energy?There are several characteristics/properties associated with microscopes. Define the following terms using your text. MagnificationResolutionContrastParfocalDepth of fieldField of viewNumerical apertureTotal magnification How is it calculated?Compound light microscopesCompound light microscopes have many different components. Correctly label the following microscope using the following terms. List the function of each componentOcular Objective Rheostat (dimmer)Condenser Lever for the iris diaphragmStage ArmBase On/off switchStage clips Coarse focusFine focus Mechanical levers for the stage (2)What is the maximum magnification when using the light microscope in the lab?Fill in the following table*:Objective Magnification Total Mag Numerical ApertureLowMedHighOil*the ocular magnification is 10XResolving Power: Magnification or biological specimens is great but is useless unless the image is clear. Resolution defines the capacity of an optical system to distinguish two points as separate or distinct. The equation for the determination of the resolving power is:Wavelength of light in nm2 X N.A. of obj lensCalculate the resolving power of your lens. 10X40X100XIn your own words what does this number mean to you? What are the units of this number?Microscope exercisesPrepared slide: Choose one to draw but look at bothObelia medusa or Human BoneField of View: Change to a higher objective. What happens to the field of view? Does it increase or decrease? Depth of field: Remember that organisms are 3 dimensional even though they are preserved in a two dimensional surface. There exists are slight depth to the prepared organisms. While under magnification, rotate slightly the fine focus to observe different layers in the organismIs this organism or tissue sample from an autotroph or heterotroph? ______________. Does this organism have a cell wall? _________. Is this multicellular or single celled?_________________________. Is this organism prokaryotic or eukaryotic? ___________________. If you have chosen the medusa please draw one specimen and draw what you see under medium magnification. If you have chosen the bone tissue you do not need to draw the entire sample, just draw one or two detailed ring structures under medium magnification.Wet Mount slide: In this class you will be asked to prepare slides of live organisms. It is important to remember when preparing slides, less is more. The thicker the specimen the more difficulty you will have focusing the scope, due to the increase in the depth of field. Prepare a wet mount of the elodea leaf.Field of View: Change to a higher objective. What happens to the field of view? Does it increase or decrease? Depth of field: Remember that organisms are 3 dimensional even though they are preserved in a two dimensional surface. There exists are slight depth to the prepared organisms. While under magnification, rotate slightly the fine focus to observe different layers in the organismDraw what you see. Is this organism an autotroph or a heterotroph? Does this organism have a cell wall___________. Label the following: cell wall, chloroplasts, cytoplasm. Draw clearly one or two cells. Label the total