J210 Practical WorksheetModule 41. Discuss bacterial growth under the following conditions. Use ‘++’, ‘+’, or ‘-’ to indicategrowth or absence of growth.Aerobic / Anaerobic conditionsa. Obligate aerobe sample -b. Facultative anaerobe samplec. Obligate anaerobe sample2. How are obligate anaerobes cultured in the laboratory using both liquid culture and agarplates?a. Liquid culture-b. Agar plates-3. Phenol red sugar fermentation tubes are used to determine if a pure colony of bacteriacan ferment a specific sugar (eg. glucose or lactose). How would you determine thefollowing outcomes?a. Production of an acid-b. Production of a gas-4. Briefly describe the role of sugar fermentation in the etiology of dental caries (asdiscussed in Lecture 3).5. Provide two reasons why sugary soda drinks increase the risk of dental caries?- It can break down the enamel of the teeth.- The carbonation, sugar, and acids encourage the growth of bacteria in your mouth andon your teeth that result in cavities.6. Why do specific bacterial species release hydrogen sulfide (H2S)? Explain the procedure(culture medium and outcome) to determine this.7. What type of bacterial enzyme would cause the liquefaction of gelatin? Where is thisenzyme made and where does it function (inside or outside the cell)?8. What is an anaerobic jar, and how is it used?- An anaerobic jar is an instrument used in the production of an anaerobic environment.It’s used to improve the quality and consistency of your bacterial cultivation.9. Discuss how technicians might use biochemical parameters (such as sugar fermentation,production of H2S, gelatin liquefaction, etc), together with a flow chart to speciatebacterial
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